Thimerosal Induces Apoptosis and G2/M Phase Arrest in Human Leukemia Cells

Kyung Jin WooTae-Jin LeeJae Hoon BaeByeong-Churl JangDae-Kyu SongJae-We ChoSeong-Il SuhJong-Wook ParkTaeg Kyu Kwon
Dept. of Immunology (면역학); Dept. of Physiology (생리학); Dept. of Molecular Medicine (분자의학); Dept. of Dermatology (피부과학); Dept. of Microbiology (미생물학); Institute for Medical Science (의과학연구소)
Issue Date
Molecular Carcinogenesis, Vol.45(9) : 657-666, 2006
Thimerosal is an organomercury compo und with su lfhydryl-reactive p roperties. The abili ty of thime rosal to act as a sulfhydryl group is related to the presence of mercury. D ue to its antibacterial effect, thimerosal is widely used a s preservatives and has been rep orte d to cause chemicall y medi ated side effects. In the present s tudy, we sh owed that the molecular mechanism of thimero sal induced apoptosi s in U937 cells. Thimerosal was sho wn to be responsible for the i nhibition of U 937 cells growth by induc ing apopt os is. Treatment with 2. 5–5 mM thi merosal but not th iosalicylic acid (structural analog of thim erosal dev oid o f mercury) for 1 2 h produce d apoptos is, G 2 /M phase ar rest, and DNA fragmen tation in a dose-dependent manner. Treatment with caspase in hibitor si gnificantly reduced thi merosal- induced caspase 3 a ctivation. In addition, th im erosa l-induced apoptosis was attenuat ed by antioxidant Mn (III) meso- te traki s (4 -benzoic acid) porphyrin (Mn-TBA P). Thes e dat a indicate that the cy totoxic effect of thimerosal on U937 cells is attributable to the induced apopt os is and that thimerosal-induced apop tosis is mediated by rea ctive o xygen species generation and caspase-3 ac ti vation. ß 2006 Wiley-Liss, Inc. Key words: thimerosa l; apoptosis; ROS; cell cycl e; cas pase; U 937 c ells
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1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Dermatology (피부과학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. Journal Papers (연구논문) > 3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
Keimyung Author(s)
박종욱; 권택규; 배재훈; 송대규; 장병철; 조재위; 서성일
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