Thimerosal Induces Apoptosis and G2/M Phase Arrest in Human Leukemia Cells
- Author(s)
- Kyung Jin Woo; Tae-Jin Lee; Jae Hoon Bae; Byeong-Churl Jang; Dae-Kyu Song; Jae-We Cho; Seong-Il Suh; Jong-Wook Park; Taeg Kyu Kwon
- Keimyung Author(s)
- Park, Jong Wook; Kwon, Taeg Kyu; Bae, Jae Hoon; Song, Dae Kyu; Jang, Byeong Churl; Cho, Jae We; Suh, Seong Il
- Department
- Dept. of Immunology (면역학)
Dept. of Physiology (생리학)
Dept. of Molecular Medicine (분자의학)
Dept. of Dermatology (피부과학)
Dept. of Microbiology (미생물학)
Institute for Medical Science (의과학연구소)
- Journal Title
- Molecular Carcinogenesis
- Issued Date
- 2006
- Volume
- 45
- Issue
- 9
- Abstract
- Thimerosal is an organomercury compo und with su lfhydryl-reactive p roperties. The abili ty of thime rosal to act as a
sulfhydryl group is related to the presence of mercury. D ue to its antibacterial effect, thimerosal is widely used a s
preservatives and has been rep orte d to cause chemicall y medi ated side effects. In the present s tudy, we sh owed that
the molecular mechanism of thimero sal induced apoptosi s in U937 cells. Thimerosal was sho wn to be responsible for
the i nhibition of U 937 cells growth by induc ing apopt os is. Treatment with 2. 5–5 mM thi merosal but not th iosalicylic
acid (structural analog of thim erosal dev oid o f mercury) for 1 2 h produce d apoptos is, G
2
/M phase ar rest, and DNA
fragmen tation in a dose-dependent manner. Treatment with caspase in hibitor si gnificantly reduced thi merosal-
induced caspase 3 a ctivation. In addition, th im erosa l-induced apoptosis was attenuat ed by antioxidant Mn (III) meso-
te traki s (4 -benzoic acid) porphyrin (Mn-TBA P). Thes e dat a indicate that the cy totoxic effect of thimerosal on U937 cells
is attributable to the induced apopt os is and that thimerosal-induced apop tosis is mediated by rea ctive o xygen species
generation and caspase-3 ac ti vation.
ß 2006 Wiley-Liss, Inc.
Key words: thimerosa l; apoptosis; ROS; cell cycl e; cas pase; U 937 c ells
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