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The Effect of Centrifugation Condition on Mature Adipocytes and Adipose Stem Cell Viability

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Author(s)
손대구김준형한기환
Alternative Author(s)
Son, Dae GuKim, Jun HyungHan, Ki Hwan
Issued Date
2014
Abstract
Different researchers have recommended different lipoaspirate centrifugation
speeds and times, probably due to the limits in fat cell viability assays.
We assessed fat cell viability using a fluorescein diacetate and propidium iodide
(FDA-PI) stain and 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-
5-carboxanilide (XTT) assay after harvesting syringe liposuction and spun with
different centrifugation speeds to determine the optimal conditions. Lipoaspirates,
harvested from 13 donors, were transferred into a centrifuge tube and spun at
1000, 3000, and 4000 rpm for 3 minutes. Mature adipocytes and adipose stem
cells were isolated and tested with a direct counting of FDA-PI-stained cells under
fluorescence microscope and XTT assay. We incubated adipocytes and adipose
stem cells for 1 day and 3 days, and we compared both of them with fresh
samples to evaluate the influence of culturing condition on fat cell viability. Centrifugation
speeds from 1000 rpm to 4000 rpm for 3 minutes showed no change
in the percentage of adipocytes and adipose stem cell viability not only in the
fresh samples but also in the cultured samples (1 day and 3 days). Centrifugation
speeds under 4000 rpm do not change the percentage of fat cell viability. To differentiate
viable cells from dying or dead mature adipocytes and oil accurately, combinations
of viability tests are essential.
Key Words: fat injection, lipofilling, adipocyte, centrifugation
Department
Dept. of Plastic Surgery (성형외과학)
Publisher
School of Medicine
Citation
Annals of Plastic Surgery, Vol.72(5) : 589-593, 2014
Type
Article
ISSN
0148-7043
DOI
10.1097/SAP.0b013e318268a85d
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35025
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Plastic Surgery (성형외과학)
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