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Viability of Fat Cells Over Time After Syringe Suction Lipectomy The Effects of Cryopreservation

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Author(s)
Daegu SonJaehoon OhTaehyun ChoiJunhyung KimKihwan HanSeongyun HaKyungho Lee
Publication Year
2010
Abstract
The purpose of this study was to determine the late decline in
viability of fat cells over time for fat tissue stored at 15°C and 70°C after
harvest from abdominal liposuction. A total of 16 females were recruited for
this study. The viability of fat cell specimens was measured after freezing for
1, 3, 7, 14, 28, and 56 days. A number of viable mature adipocytes were
evaluated by fluorescence microscopy after staining with fluorescein diacetate
and propidium iodide. The glycerol-3-phosphate dehydrogenase activity
was measured in lipoaspirates before digestion and the XTT reduction
assay was performed. In addition, the XTT reduction assay was also
performed on isolated lipocytes and preadipocytes.
The viability of mature adipocytes was very low for both the 15°C and
70°C samples after 1 day of freezing (13.3% 7.4% and 12.6% 6.3%,
respectively). There was no statistically significant difference between the
samples stored at the 2 temperatures. The GPDH activity of the lipoaspirates
frozen, for 1 day, at 15°C and 70°C was 25.1% 10% and 28.7%
11%, respectively. For the XTT test, the fractional enzyme activity of the
lipoaspirates frozen, for 1 day, at 15°C and 70°C was 30.0% 10.9%
and 36.1% 12.3%, respectively. In addition, the adipocytes had low
activity from day one: 15.4% 7.2% at 15°C and 11.5% 5.6% at
70°C. Furthermore, the preadipocytes had a low activity of 8.0% 6.0%
at 15°C and 8.6% 3.8% at 70°C. At 8 weeks, there were few viable
mature adipocytes and the activity of the cells was very low by XTT and
GPDH testing.
The results of this study showed that the viability of adipocytes declined
rapidly after frozen storage for 1 day at both 15°C and 70°C, and
decreased gradually in storage after 8 weeks; at which time only approximately
5% of the fat cells were alive. These findings suggest that the present
fat preservation storage techniques using a 15°C freezer or a 70°C deep
freezer are both inadequate to maintain the viability of fat cells.
Key Words: fat viability, cryopreservation, fat graft
Department
Dept. of Plastic Surgery (성형외과학)
Publisher
School of Medicine
Citation
Annals of Plastic Surgery, Vol.65(3) : 354-360, 2010
Type
Article
ISSN
0148-7043
DOI
10.1097/SAP.0b013e3181bb49b8
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35027
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