계명대학교 의학도서관 Repository

Chloroquine Has a Cytotoxic Effect on Acanthamoeba Encystation through Modulation of Autophagy

Metadata Downloads
Author(s)
김현아서성일서민호백원기
Alternative Author(s)
Kim, Hyun AhSuh, Seong IlSuh, Min HoBaek, Won Ki
Publication Year
2014
Abstract
Encystation of Acanthamoeba castellanii is associated with resistance to chemotherapeutic agents. Blocking the encystation process could potentiate the efficacy of chemotherapeutic agents and biocides. During encystation, autophagy is highly stimulated and required for proper encystation of Acanthamoeba. In this study, the cytotoxic effect of chloroquine, a well-known autophagy-inhibitory drug, was tested in A. castellanii. Chloroquine was able to selectively reduce cell survival during the encystation of A. castellanii. However, A. castellanii trophozoites and mature cysts were resistant to chloroquine. Chloroquine treatment led to an increase in the number and size of lysosomes in encysting cells. Moreover, chloroquine inhibited the degradation of long-lived proteins in the encysting cells. Decreased autophagic flux, indicated by an increased number of lysosomes and decreased degradation of long-lived proteins, may be the mechanism by which cell death is induced by chloroquine in encysting Acanthamoeba. These results suggest a potential novel therapeutic application of chloroquine as an anti-Acanthamoeba drug. Our findings also suggest that targeting autophagy could be a therapeutic strategy against Acanthamoeba infection.
Department
Dept. of Internal Medicine (내과학)
Dept. of Microbiology (미생물학)
Publisher
School of Medicine
Citation
Antimicrobial Agents and Chemotherapy, Vol.58(10) : 6235-6241, 2014
Type
Article
ISSN
0066-4804
DOI
10.1128/AAC.03164-14
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35063
Authorize & License
  • AuthorizeOpen
  • EmbargoForever
Files in This Item:

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.