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Chloroquine Has a Cytotoxic Effect on Acanthamoeba Encystation through Modulation of Autophagy

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Author(s)
Bijay Kumar JhaHui-Jung JungIncheol SeoHyun Ah KimSeong-Il SuhMin-Ho SuhWon-Ki Baek
Keimyung Author(s)
Kim, Hyun AhSuh, Seong IlSuh, Min HoBaek, Won Ki
Department
Dept. of Internal Medicine (내과학)
Dept. of Microbiology (미생물학)
Journal Title
Antimicrobial Agents and Chemotherapy
Issued Date
2014
Volume
58
Issue
10
Abstract
Encystation of Acanthamoeba castellanii is associated with resistance to chemotherapeutic agents. Blocking the encystation process could potentiate the efficacy of chemotherapeutic agents and biocides. During encystation, autophagy is highly stimulated and required for proper encystation of Acanthamoeba. In this study, the cytotoxic effect of chloroquine, a well-known autophagy-inhibitory drug, was tested in A. castellanii. Chloroquine was able to selectively reduce cell survival during the encystation of A. castellanii. However, A. castellanii trophozoites and mature cysts were resistant to chloroquine. Chloroquine treatment led to an increase in the number and size of lysosomes in encysting cells. Moreover, chloroquine inhibited the degradation of long-lived proteins in the encysting cells. Decreased autophagic flux, indicated by an increased number of lysosomes and decreased degradation of long-lived proteins, may be the mechanism by which cell death is induced by chloroquine in encysting Acanthamoeba. These results suggest a potential novel therapeutic application of chloroquine as an anti-Acanthamoeba drug. Our findings also suggest that targeting autophagy could be a therapeutic strategy against Acanthamoeba infection.
Keimyung Author(s)(Kor)
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Publisher
School of Medicine
Citation
Bijay Kumar Jha et al. (2014). Chloroquine Has a Cytotoxic Effect on Acanthamoeba Encystation through Modulation of Autophagy. Antimicrobial Agents and Chemotherapy, 58(10), 6235–6241. doi: 10.1128/AAC.03164-14
Type
Article
ISSN
0066-4804
Source
http://aac.asm.org/content/58/10/6235
DOI
10.1128/AAC.03164-14
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35063
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
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