Evaluation of an Amniotic Membrane-Collagen Dermal Substitute in the Management of Full-Thickness Skin Defects in a Pig
- 손대구; 김준형; 한기환; 권선영
- Alternative Author(s)
- Son, Dae Gu; Kim, Jun Hyung; Han, Ki Hwan; Kwon, Sun Young
- Publication Year
- Background To minimize the inflammatory reaction and improve healing, a new modified
dermal substitute composed of an atelocollagen, chondroitin-6-sulfate, and amniotic
membrane (AM) was applied to full-thickness skin defects in a pig. Atelocollagen was extracted
from bovine skin, and two modified dermal substitutes were generated according to the crosslinking
Methods The AM-collagen dermal substitutes were characterized and compared with
currently used dermal substitutes in a pig skin defect model. There were five experimental
groups: dehydrothermal (DHT) cross-linking atelocollagen with the AM on the top (AM-DHT),
DHT and chemical cross-linking atelocollagen with the AM on the top (AM-DHT/chemical),
Terudermis, Integra, and AlloDerm. After 3×3 cm full-thickness skin defects on the back of
a pig were created, each dermal substitutes dermal substitutes was randomly grafted on the
defects. Two weeks after grafting, autologous partial-thickness skin was over-grafted on the
neodermis. The take rate of the dermal substitutes, skin, and histological sections were all
assessed at 1, 2, and 4 weeks postoperatively.
Results More rapid healing and a higher take rate were evident in the AM-DHT and Terudermis
groups. Histological examination revealed fewer inflammatory cells and more fibroblast
hyperplasia in these two groups. Four weeks after surgery, the amount of newly formed
collagen was significantly more appropriate in the AM-DHT group.
Conclusions These observations provide supporting evidence that a newly developed amnioticcollagen
dermal substitute may inhibit inflammatory reactions and promote wound healing.
Keywords Amnion / Dermis / Biologic dressings / Collagen / Chondroitin sulfates
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