EGCG Attenuates AMPA-Induced Intracellular Calcium Increase in Hippocampal Neurons

Authors
Jae Hoon BaeKyo Cheol MunWon Kyun ParkSeung-Ryong LeeSeong-Il SuhWon Ki BaekMan-Bin YimTaeg Kyu KwonDae-Kyu Song
Department
Dept. of Physiology (생리학); Dept. of Biochemistry (생화학); Dept. of Immunology (면역학); Dept. of Neurosurgery (신경외과학); Dept. of Microbiology (미생물학); Dept. of Pharmacology (약리학); Brain Research Institute (뇌연구소); Dept. of Medical Education (의학교육학)
Issue Date
2002
Citation
Biochemical and Biophysical Research Communications, Vol.290(5) : 1506-1512, 2002
ISSN
0006-291X
Abstract
We have investigated the protective effect of (−)-epigallocatechin gallate (EGCG) on α-amino-3-hydroxy-5-methyl-4-isoxazolo propionate (AMPA)-induced toxicity in cultured rat hippocampal neurons. Treatment of 24 h AMPA (10 μM) reduced the neuronal viability in both survival neuron counting and MTT reduction assay compared with control, with increase in cellular concentrations of hydrogen peroxide and malondialdehyde. These responses to AMPA were significantly blocked by co-treatments with EGCG (10 μM), which effect was very similar to the protective ability of a known antioxidant catalase (2000 U/ml). AMPA (50 μM) elicited the increase in intracellular calcium concentration ([Ca2+]i) on which EGCG significantly attenuated both peak amplitude and sustained nature of that [Ca2+]i increase in a dose-dependent manner. These data suggest that EGCG has a neuroprotective effect against AMPA through inhibition of AMPA-induced [Ca2+]i increase and consequent attenuation of reactive oxygen species production and lipid peroxidation as an antioxidant and a radical scavenger. Keywords hippocampal neurons; (−)-epigallocatechin gallate; AMPA; intracellular calcium concentration; hydrogen peroxide; malondialdehyde
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35145
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Pharmacology (약리학)
1. Journal Papers (연구논문) > 3. Research Institutues (연구소) > Brain Research Institute (뇌연구소)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Medical Education (의학교육학)
Keimyung Author(s)
배재훈; 송대규; 문교철; 권택규; 임만빈; 서성일; 백원기; 이성용; 박원균
Full Text
https://linkinghub.elsevier.com/retrieve/pii/S0006291X02963722
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