EGCG Attenuates AMPA-Induced Intracellular Calcium
Increase in Hippocampal Neurons
- Affiliated Author(s)
- 배재훈; 송대규; 문교철; 권택규; 임만빈; 서성일; 백원기; 이성용; 박원균
- Alternative Author(s)
- Bae, Jae Hoon; Song, Dae Kyu; Mun, Kyo Cheol; Kwon, Taeg Kyu; Yim, Man Bin; Suh, Seong Il; Baek, Won Ki; Lee, Seong Ryong; Park, Won Kyun
- Journal Title
- Biochemical and Biophysical Research Communications
- Issued Date
- We have investigated the protective effect of (−)-epigallocatechin gallate (EGCG) on α-amino-3-hydroxy-5-methyl-4-isoxazolo propionate (AMPA)-induced toxicity in cultured rat hippocampal neurons. Treatment of 24 h AMPA (10 μM) reduced the neuronal viability in both survival neuron counting and MTT reduction assay compared with control, with increase in cellular concentrations of hydrogen peroxide and malondialdehyde. These responses to AMPA were significantly blocked by co-treatments with EGCG (10 μM), which effect was very similar to the protective ability of a known antioxidant catalase (2000 U/ml). AMPA (50 μM) elicited the increase in intracellular calcium concentration ([Ca2+]i) on which EGCG significantly attenuated both peak amplitude and sustained nature of that [Ca2+]i increase in a dose-dependent manner. These data suggest that EGCG has a neuroprotective effect against AMPA through inhibition of AMPA-induced [Ca2+]i increase and consequent attenuation of reactive oxygen species production and lipid peroxidation as an antioxidant and a radical scavenger.
intracellular calcium concentration;
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