Pyrrolidine dithiocarbamate induces cyclooxygenase-2 expression
in NIH 3T3 fibroblast cells
- 서성일; 서민호; 백원기; 권택규; 박종욱; 배재훈; 송대규; 조치흠
- Alternative Author(s)
- Suh, Seong Il; Suh, Min Ho; Baek, Won Ki; Kwon, Taeg Kyu; Park, Jong Wook; Bae, Jae Hoon; Song, Dae Kyu; Cho, Chi Heum
- Publication Year
- Pyrrolidine dithiocarbamate (PDTC) is a metal chelating compound that can exert either pro-oxidant or antioxidant effects in
different situations. Several studies demonstrate that it can inhibit cyclooxygenase-2 (COX-2) expression, which may be due to its
antioxidant activity. Here, we found that PDTC rather increased COX-2 expression in NIH 3T3. The increase of COX-2 expression
was inhibited by adding bathocuproline disulfonic acid, a non-permeable specific copper chelator, in the incubation medium. This
result suggests that PDTC exerts its effect by transporting redox-active copper ions into the cells. In support of this observation,
PDTC did not induce COX-2 expression in a serum-free environment. When PDTC was added with copper in the serum-free
medium, it acted as the inducer of COX-2 expression. In addition, pretreatment of N-acetyl-L-cystein or dithiothreitol, other antioxidants,
inhibited the PDTC-induced COX-2 expression. Our data indicate that PDTC induces COX-2 expression in NIH 3T3
cells, which may be due to its activities as a copper chelator and a pro-oxidant.
2002 Elsevier Science (USA). All rights reserved.
Keywords: Pyrrolidine dithiocarbamate; Cyclooxygenase-2; Copper; Pro-oxidant; Fibroblast
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