Pyrrolidine dithiocarbamate induces cyclooxygenase-2 expression in NIH 3T3 fibroblast cells

Jeong Eun LeeKyung Min KimJae-We ChoSeong-Il SuhMin-Ho SuhTaeg Kyu KwonJong-Wook ParkJae Hoon BaeDae-Kyu SongChi-Heum ChoInsoo BaeWon-Ki Baek
Dept. of Microbiology (미생물학); Dept. of Immunology (면역학); Dept. of Physiology (생리학); Dept. of Obstetrics & Gynecology (산부인과학); Institute for Medical Science (의과학연구소)
Issue Date
Biochemical and Biophysical Research Communications, Vol.298(2) : 230-234, 2002
Pyrrolidine dithiocarbamate (PDTC) is a metal chelating compound that can exert either pro-oxidant or antioxidant effects in different situations. Several studies demonstrate that it can inhibit cyclooxygenase-2 (COX-2) expression, which may be due to its antioxidant activity. Here, we found that PDTC rather increased COX-2 expression in NIH 3T3. The increase of COX-2 expression was inhibited by adding bathocuproline disulfonic acid, a non-permeable specific copper chelator, in the incubation medium. This result suggests that PDTC exerts its effect by transporting redox-active copper ions into the cells. In support of this observation, PDTC did not induce COX-2 expression in a serum-free environment. When PDTC was added with copper in the serum-free medium, it acted as the inducer of COX-2 expression. In addition, pretreatment of N-acetyl-L-cystein or dithiothreitol, other antioxidants, inhibited the PDTC-induced COX-2 expression. Our data indicate that PDTC induces COX-2 expression in NIH 3T3 cells, which may be due to its activities as a copper chelator and a pro-oxidant. 2002 Elsevier Science (USA). All rights reserved. Keywords: Pyrrolidine dithiocarbamate; Cyclooxygenase-2; Copper; Pro-oxidant; Fibroblast
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1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Obstetrics & Gynecology (산부인과학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
1. Journal Papers (연구논문) > 3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
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서성일; 서민호; 백원기; 권택규; 박종욱; 배재훈; 송대규; 조치흠
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