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Pyrrolidine dithiocarbamate induces cyclooxygenase-2 expression in NIH 3T3 fibroblast cells

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Author(s)
서성일서민호백원기권택규박종욱배재훈송대규조치흠
Alternative Author(s)
Suh, Seong IlSuh, Min HoBaek, Won KiKwon, Taeg KyuPark, Jong WookBae, Jae HoonSong, Dae KyuCho, Chi Heum
Publication Year
2002
Abstract
Pyrrolidine dithiocarbamate (PDTC) is a metal chelating compound that can exert either pro-oxidant or antioxidant effects in
different situations. Several studies demonstrate that it can inhibit cyclooxygenase-2 (COX-2) expression, which may be due to its
antioxidant activity. Here, we found that PDTC rather increased COX-2 expression in NIH 3T3. The increase of COX-2 expression
was inhibited by adding bathocuproline disulfonic acid, a non-permeable specific copper chelator, in the incubation medium. This
result suggests that PDTC exerts its effect by transporting redox-active copper ions into the cells. In support of this observation,
PDTC did not induce COX-2 expression in a serum-free environment. When PDTC was added with copper in the serum-free
medium, it acted as the inducer of COX-2 expression. In addition, pretreatment of N-acetyl-L-cystein or dithiothreitol, other antioxidants,
inhibited the PDTC-induced COX-2 expression. Our data indicate that PDTC induces COX-2 expression in NIH 3T3
cells, which may be due to its activities as a copper chelator and a pro-oxidant.
2002 Elsevier Science (USA). All rights reserved.
Keywords: Pyrrolidine dithiocarbamate; Cyclooxygenase-2; Copper; Pro-oxidant; Fibroblast
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