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Pyrrolidine dithiocarbamate induces cyclooxygenase-2 expression in NIH 3T3 fibroblast cells

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Author(s)
Jeong Eun LeeKyung Min KimJae-We ChoSeong-Il SuhMin-Ho SuhTaeg Kyu KwonJong-Wook ParkJae Hoon BaeDae-Kyu SongChi-Heum ChoInsoo BaeWon-Ki Baek
Keimyung Author(s)
Suh, Seong IlSuh, Min HoBaek, Won KiKwon, Taeg KyuPark, Jong WookBae, Jae HoonSong, Dae KyuCho, Chi Heum
Department
Dept. of Microbiology (미생물학)
Dept. of Immunology (면역학)
Dept. of Physiology (생리학)
Dept. of Obstetrics & Gynecology (산부인과학)
Institute for Medical Science (의과학연구소)
Journal Title
Biochemical and Biophysical Research Communications
Issued Date
2002
Volume
298
Issue
2
Abstract
Pyrrolidine dithiocarbamate (PDTC) is a metal chelating compound that can exert either pro-oxidant or antioxidant effects in
different situations. Several studies demonstrate that it can inhibit cyclooxygenase-2 (COX-2) expression, which may be due to its
antioxidant activity. Here, we found that PDTC rather increased COX-2 expression in NIH 3T3. The increase of COX-2 expression
was inhibited by adding bathocuproline disulfonic acid, a non-permeable specific copper chelator, in the incubation medium. This
result suggests that PDTC exerts its effect by transporting redox-active copper ions into the cells. In support of this observation,
PDTC did not induce COX-2 expression in a serum-free environment. When PDTC was added with copper in the serum-free
medium, it acted as the inducer of COX-2 expression. In addition, pretreatment of N-acetyl-L-cystein or dithiothreitol, other antioxidants,
inhibited the PDTC-induced COX-2 expression. Our data indicate that PDTC induces COX-2 expression in NIH 3T3
cells, which may be due to its activities as a copper chelator and a pro-oxidant.
2002 Elsevier Science (USA). All rights reserved.
Keywords: Pyrrolidine dithiocarbamate; Cyclooxygenase-2; Copper; Pro-oxidant; Fibroblast
Keimyung Author(s)(Kor)
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Publisher
School of Medicine
Citation
Jeong Eun Lee et al. (2002). Pyrrolidine dithiocarbamate induces cyclooxygenase-2 expression
in NIH 3T3 fibroblast cells. Biochemical and Biophysical Research Communications, 298(2), 230–234. doi: 10.1016/S0006-291X(02)02430-0
Type
Article
ISSN
0006-291X
Source
https://linkinghub.elsevier.com/retrieve/pii/S0006291X02024300
DOI
10.1016/S0006-291X(02)02430-0
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35147
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. School of Medicine (의과대학) > Dept. of Obstetrics & Gynecology (산부인과학)
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
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