Transcriptional repression of E2F gene by proteasome inhibitors in human osteosarcoma cells

Authors
Jun Hee LimYoung-Chae ChangYong Bok ParkJong-Wook ParkTaeg Kyu Kwon
Department
Dept. of Immunology (면역학)
Issue Date
2004
Citation
Biochemical and Biophysical Research Communications, Vol.318(4) : 868-872, 2004
ISSN
0006-291X
Abstract
E2F family of transcription factors regulates the transcription of genes required for DNA synthesis. E2F is itself controlled by a series of transcriptional and post-transcriptional pathways. Here we provide evidence that proteasome inhibitor-mediated E2F1 gene down-regulation is regulated by transcriptional events. Using the proteasome-specific inhibitors, MG132 and lactacystin, we show that the p53, the cdk inhibitors p21 and p27, and cyclin A are degraded by the ubiquitin–proteasome pathway in human osteosarcoma cells. Interestingly, the expression levels of E2F1 and E2F2 are down-regulated by proteasome inhibitors. E2F promoter and RT-PCR assay clearly demonstrated that proteasome inhibitors could reduce E2F transcriptional activation. However, MG132-induced repression of E2F1 and E2F2 is not associated with ROS generation. Keywords Proteasome inhibitor; E2F; Ubiquitin; Transcriptional regulation; MG132
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35180
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
Keimyung Author(s)
박종욱; 권택규
Full Text
https://linkinghub.elsevier.com/retrieve/pii/S0006291X04008599
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