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Transcriptional repression of E2F gene by proteasome inhibitors in human osteosarcoma cells

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Author(s)
Jun Hee LimYoung-Chae ChangYong Bok ParkJong-Wook ParkTaeg Kyu Kwon
Keimyung Author(s)
Park, Jong WookKwon, Taeg Kyu
Department
Dept. of Immunology (면역학)
Journal Title
Biochemical and Biophysical Research Communications
Issued Date
2004
Volume
318
Issue
4
Abstract
E2F family of transcription factors regulates the transcription of genes required for DNA synthesis. E2F is itself controlled by a series of transcriptional and post-transcriptional pathways. Here we provide evidence that proteasome inhibitor-mediated E2F1 gene down-regulation is regulated by transcriptional events. Using the proteasome-specific inhibitors, MG132 and lactacystin, we show that the p53, the cdk inhibitors p21 and p27, and cyclin A are degraded by the ubiquitin–proteasome pathway in human osteosarcoma cells. Interestingly, the expression levels of E2F1 and E2F2 are down-regulated by proteasome inhibitors. E2F promoter and RT-PCR assay clearly demonstrated that proteasome inhibitors could reduce E2F transcriptional activation. However, MG132-induced repression of E2F1 and E2F2 is not associated with ROS generation.

Keywords
Proteasome inhibitor;
E2F;
Ubiquitin;
Transcriptional regulation;
MG132
Keimyung Author(s)(Kor)
박종욱
권택규
Publisher
School of Medicine
Citation
Jun Hee Lim et al. (2004). Transcriptional repression of E2F gene by proteasome inhibitors
in human osteosarcoma cells. Biochemical and Biophysical Research Communications, 318(4), 868–872. doi: 10.1016/j.bbrc.2004.04.103
Type
Article
ISSN
0006-291X
Source
https://linkinghub.elsevier.com/retrieve/pii/S0006291X04008599
DOI
10.1016/j.bbrc.2004.04.103
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35180
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
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