계명대학교 의학도서관 Repository

Inhibition of ATP-sensitive K+ channels by taurine through a benzamido-binding site on sulfonylurea receptor 1

Metadata Downloads
Author(s)
임정근백원기권택규서성일박종욱이인규배재훈송대규
Alternative Author(s)
Lim, Jeong GeunBaek, Won KiKwon, Taeg KyuSuh, Seong IlPark, Jong WookLee, In KyuBae, Jae HoonSong, Dae Kyu
Publication Year
2004
Abstract
ATP-sensitive potassium (KATP) channels in pancreatic β-cells comprise sulfonylurea receptor (SUR) 1 and inwardly-rectifying potassium channel (Kir) 6.2 subunits. We have evaluated the effect of intracellular taurine on KATP channel activity in rat pancreatic β-cells using the patch-clamp technique. The mechanism of taurine action was also examined using recombinant KATP channels. The islets and single β-cells from male Sprague–Dawley rats were collected by collagenase digestion technique. Single KATP channel currents were recorded by the inside–out mode at a membrane potential of −60 mV. Cytosolic free-Ca2+ concentration ([Ca2+]c) and insulin secretory capacity were measured by the dual-excitation fluorimetry and radioimmunoassay, respectively. The native β-cell KATP channel was directly inhibited by taurine in a dose-dependent manner. Taurine did not influence ATP-mediated inhibition or MgADP-induced activation of the channel activity. The sensitivity of the KATP channel to glybenclamide, but not gliclazide, was enhanced by taurine. Glybenclamide elicited a greater increase in [Ca2+]c and increased insulin secretion in the β-cells when pretreated with taurine. Taurine did not inhibit Kir6.2ΔC36 currents, a truncated form of Kir6.2, expressed in Xenopus oocytes without SUR. These results demonstrate that taurine inhibits the KATP channel activity in the β-cells, interacting with a benzamido-binding site on SUR1, but not Kir6.2. Keywords
Taurine;
ATP-sensitive potassium channel;
Sulfonylurea;
Ca2+ concentration;
Insulin;
Pancreatic β-cell
Authorize & License
  • AuthorizeOpen
  • EmbargoForever
Files in This Item:

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.