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Inhibition of ATP-sensitive K+ channels by taurine through a benzamido-binding site on sulfonylurea receptor 1

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Author(s)
Eun-Jee ParkJae Hoon BaeSo-Yeon KimJeong-Geun LimWon-Ki BaekTaeg Kyu KwonSeong-il SuhJong-Wook ParkIn-Kyu LeeFrances M. AshcroftDae-Kyu Song
Keimyung Author(s)
Lim, Jeong GeunBaek, Won KiKwon, Taeg KyuSuh, Seong IlPark, Jong WookLee, In KyuBae, Jae HoonSong, Dae Kyu
Department
Dept. of Physiology (생리학)
Dept. of Neurology (신경과학)
Dept. of Microbiology (미생물학)
Dept. of Immunology (면역학)
Dept. of Internal Medicine (내과학)
Journal Title
Biochemical Pharmacology
Issued Date
2004
Volume
67
Issue
6
Abstract
ATP-sensitive potassium (KATP) channels in pancreatic β-cells comprise sulfonylurea receptor (SUR) 1 and inwardly-rectifying potassium channel (Kir) 6.2 subunits. We have evaluated the effect of intracellular taurine on KATP channel activity in rat pancreatic β-cells using the patch-clamp technique. The mechanism of taurine action was also examined using recombinant KATP channels. The islets and single β-cells from male Sprague–Dawley rats were collected by collagenase digestion technique. Single KATP channel currents were recorded by the inside–out mode at a membrane potential of −60 mV. Cytosolic free-Ca2+ concentration ([Ca2+]c) and insulin secretory capacity were measured by the dual-excitation fluorimetry and radioimmunoassay, respectively. The native β-cell KATP channel was directly inhibited by taurine in a dose-dependent manner. Taurine did not influence ATP-mediated inhibition or MgADP-induced activation of the channel activity. The sensitivity of the KATP channel to glybenclamide, but not gliclazide, was enhanced by taurine. Glybenclamide elicited a greater increase in [Ca2+]c and increased insulin secretion in the β-cells when pretreated with taurine. Taurine did not inhibit Kir6.2ΔC36 currents, a truncated form of Kir6.2, expressed in Xenopus oocytes without SUR. These results demonstrate that taurine inhibits the KATP channel activity in the β-cells, interacting with a benzamido-binding site on SUR1, but not Kir6.2. Keywords
Taurine;
ATP-sensitive potassium channel;
Sulfonylurea;
Ca2+ concentration;
Insulin;
Pancreatic β-cell
Keimyung Author(s)(Kor)
임정근
백원기
권택규
서성일
박종욱
이인규
배재훈
송대규
Publisher
School of Medicine
Citation
Eun-Jee Park et al. (2004). Inhibition of ATP-sensitive K+ channels by taurine through
a benzamido-binding site on sulfonylurea receptor 1. Biochemical Pharmacology, 67(6), 1089–1096. doi: 10.1016/j.bcp.2003.11.003
Type
Article
ISSN
0006-2952
Source
https://www.sciencedirect.com/science/article/pii/S0006295203008852?via%3Dihub
DOI
10.1016/j.bcp.2003.11.003
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35188
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. School of Medicine (의과대학) > Dept. of Neurology (신경과학)
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
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