Up-regulation of acid-sensing ion channels in the capsule of the joint in frozen shoulder

Authors
C. H. ChoY. M. LhoE. HaI. HwangK. S. SongB. W. MinK. C. BaeD. H. Kim
Department
Dept. of Orthopedic Surgery (정형외과학); Dept. of Biochemistry (생화학); Dept. of Pathology (병리학); Dept. of Rehabilitation Medicine (재활의학)
Issue Date
2015
Citation
Bone & Joint Journal, Vol.97-B(6) : 824-829, 2015
ISSN
2049-4394
Abstract
The purpose of this study was to evaluate the expression of acid-sensing ion channels (ASICs) in the capsule and synovial fluid of patients with frozen shoulder. Capsular tissue and synovial fluid were obtained from 18 patients with idiopathic frozen shoulder (FS group) and 18 patients with instability of the shoulder (control group). The expressions of ASIC1, ASIC2, and ASIC3 in the capsule were determined using the reverse transcriptase-polymerase chain reaction, immunoblot analysis, and immunohistochemistry (IHC). The concentrations in synovial fluid were evaluated using an enzyme-linked immunosorbent assay. The mRNA expression of ASIC1, ASIC2 and ASIC3 in the capsule were significantly increased in the FS group compared with the control group. The protein levels of these three ASICs were also increased. The increased expressions were confirmed by IHC. Of the ASICs, ASIC3 showed the greatest increase in both mRNA and levels of expression compared with the control group. The levels of ASIC1 and ASIC3 in synovial fluid were significantly increased in the FS group. This study suggests that ASICs may play a role as mediators of inflammatory pain and be involved in the pathogenesis of frozen shoulder.
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35252
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Rehabilitation Medicine (재활의학)
Keimyung Author(s)
조철현; 송광순; 민병우; 배기철; 하은영; 황일선; 김두환
Full Text
https://online.boneandjoint.org.uk/doi/full/10.1302/0301-620X.97B6.35254
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