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Genetic instability in the human lymphocyte exposed to hypoxia

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Author(s)
이재호최인장김대광송대규
Alternative Author(s)
Lee, Jae HoChoi, In JangKim, Dae KwangSong, Dae Kyu
Publication Year
2010
Abstract
Hypoxia, one of the key tumor microenviromental factors, promotes genetic instability, which is the hallmark of human cancers. Many recent studies have demonstrated that hypoxia by itself can lead to conditions that elevate mutagenesis and inhibit the DNA repair process in cancer. The aim of this study was to investigate the cytogenetic damage and DNA repair functions in human peripheral lymphocytes exposed to hypoxia by means of sister chromatid exchange and nuclear and mitochondrial microsatellite instability (nMSI and mtMSI), respectively. Primary lymphocyte cultures obtained from blood samples of 40 healthy donors were exposed to hypoxia for 12 and 24 hours. Genomic DNA was then isolated from the fixed lymphocytes to analyze the DNA repair process by nMSI and mtMSI. The present results revealed gradual increases in SCE for both exposure times, compared to the controls, but there was no significant correlation between hypoxia and MSI. The SCE assay showed that hypoxia by itself may induce mutagenesis by causing DNA damage in normal cells. However, the DNA repair function through MSI analysis was intact.
Department
Dept. of Anatomy (해부학)
Dept. of Medical Genetics (의학유전학)
Dept. of Physiology (생리학)
Publisher
School of Medicine
Citation
Cancer Genetics and Cytogenetics, Vol.196(1) : 83-88, 2010
Type
Article
ISSN
0165-4608
DOI
10.1016/j.cancergencyto.2009.09.002
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35308
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