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Genetic instability in the human lymphocyte exposed to hypoxia

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Author(s)
Jae-Ho LeeIn-Jang ChoiDae-Kyu SongDae-Kwang Kim
Keimyung Author(s)
Lee, Jae HoChoi, In JangKim, Dae KwangSong, Dae Kyu
Department
Dept. of Anatomy (해부학)
Dept. of Medical Genetics (의학유전학)
Dept. of Physiology (생리학)
Journal Title
Cancer Genetics and Cytogenetics
Issued Date
2010
Volume
196
Issue
1
Abstract
Hypoxia, one of the key tumor microenviromental factors, promotes genetic instability, which is the hallmark of human cancers. Many recent studies have demonstrated that hypoxia by itself can lead to conditions that elevate mutagenesis and inhibit the DNA repair process in cancer. The aim of this study was to investigate the cytogenetic damage and DNA repair functions in human peripheral lymphocytes exposed to hypoxia by means of sister chromatid exchange and nuclear and mitochondrial microsatellite instability (nMSI and mtMSI), respectively. Primary lymphocyte cultures obtained from blood samples of 40 healthy donors were exposed to hypoxia for 12 and 24 hours. Genomic DNA was then isolated from the fixed lymphocytes to analyze the DNA repair process by nMSI and mtMSI. The present results revealed gradual increases in SCE for both exposure times, compared to the controls, but there was no significant correlation between hypoxia and MSI. The SCE assay showed that hypoxia by itself may induce mutagenesis by causing DNA damage in normal cells. However, the DNA repair function through MSI analysis was intact.
Keimyung Author(s)(Kor)
이재호
최인장
김대광
송대규
Publisher
School of Medicine
Citation
Jae-Ho Lee et al. (2010). Genetic instability in the human lymphocyte exposed to hypoxia. Cancer Genetics and Cytogenetics, 196(1), 83–88. doi: 10.1016/j.cancergencyto.2009.09.002
Type
Article
ISSN
0165-4608
Source
https://www.sciencedirect.com/science/article/pii/S0165460809005196?via%3Dihub
DOI
10.1016/j.cancergencyto.2009.09.002
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35308
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Anatomy (해부학)
1. School of Medicine (의과대학) > Dept. of Medical Genetics (의학유전학)
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
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