TGF-ß1-treated ADSCs-CM promotes expression of type Ⅰ collagen and MMP-1, migration of human skin fibroblasts, and wound healing in vitro and in vivo

Authors
JAE-WE CHOMIN-CHUL KANGKYU-SUK LEE
Department
Dept. of Dermatology (피부과학); Institute for Medical Science (의과학연구소)
Issue Date
2010
Citation
International Journal of Molecular Medicine, Vol.26(6) : 901-906, 2010
ISSN
1107-3756
Abstract
. Conditioned medium from adipose-derived stem cells (ADSCs) stimulates both collagen synthesis and migration of dermal fibroblasts. However, it is still unknown whether conditioned media from tumor growth factor (TGF)-ß1-treated ADSCs (TGF-ß1-treated ADSCs-CM) induces increased expression of type I collagen, matrix metalloproteinase-1 (MMP-1), and migration as well as cell cycle regulatory proteins in fibroblasts, compared to non-treated ADSCs-CM. Our data showed that TGF-ß1-treated ADSCs-CM promoted effectively the proliferation and migration of human skin fibroblasts, compared to non-treated ADSCs-CM. In addition the expression of MMP-1 were markedly increased by treatment of TGF-ß1-treated ADSCs-CM in fibroblasts, compared to non-treated ADSCs-CM. Expression of type I collagen protein were slightly increased by treatment of TGF-ß1-treated ADSCs-CM in fibroblasts. The expression of cell cycle regulators of G1/S phase transition were not markedly altered by treatment of TGF-ß1-treated ADSCs-CM. Finally, artificial wounds were made using a 4-mm punch biopsy in hairless mice and TGF-ß1-treated ADSCs-CM were injected into the wound area. The injection of TGF-ß1-treated ADSCs- CM promoted the wound healing process in hairless mice. Taken together, our data indicated that TGF-ß1-treated ADSCs-CM induced up-regulation of type I collagen and MMP-1, promoted the migration of skin fibroblasts, and thereby promoted the wound healing process in vivo. Our data indicate that TGF-ß1-treated ADSCs-CM will be a component for a wound healing accelerating agent.
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35963
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Dermatology (피부과학)
1. Journal Papers (연구논문) > 3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
Keimyung Author(s)
조재위; 이규석
Full Text
https://www.spandidos-publications.com/ijmm/26/6/901
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