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TGF-ß1-treated ADSCs-CM promotes expression of type Ⅰ collagen and MMP-1, migration of human skin fibroblasts, and wound healing in vitro and in vivo

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Author(s)
JAE-WE CHOMIN-CHUL KANGKYU-SUK LEE
Keimyung Author(s)
Cho, Jae WeLee, Kyu Suk
Department
Dept. of Dermatology (피부과학)
Institute for Medical Science (의과학연구소)
Journal Title
International Journal of Molecular Medicine
Issued Date
2010
Volume
26
Issue
6
Abstract
. Conditioned medium from adipose-derived stem
cells (ADSCs) stimulates both collagen synthesis and migration
of dermal fibroblasts. However, it is still unknown whether
conditioned media from tumor growth factor (TGF)-ß1-treated
ADSCs (TGF-ß1-treated ADSCs-CM) induces increased
expression of type I collagen, matrix metalloproteinase-1
(MMP-1), and migration as well as cell cycle regulatory
proteins in fibroblasts, compared to non-treated ADSCs-CM.
Our data showed that TGF-ß1-treated ADSCs-CM promoted
effectively the proliferation and migration of human skin
fibroblasts, compared to non-treated ADSCs-CM. In addition
the expression of MMP-1 were markedly increased by
treatment of TGF-ß1-treated ADSCs-CM in fibroblasts,
compared to non-treated ADSCs-CM. Expression of type I
collagen protein were slightly increased by treatment of
TGF-ß1-treated ADSCs-CM in fibroblasts. The expression of
cell cycle regulators of G1/S phase transition were not markedly
altered by treatment of TGF-ß1-treated ADSCs-CM. Finally,
artificial wounds were made using a 4-mm punch biopsy in
hairless mice and TGF-ß1-treated ADSCs-CM were injected
into the wound area. The injection of TGF-ß1-treated ADSCs-
CM promoted the wound healing process in hairless mice.
Taken together, our data indicated that TGF-ß1-treated
ADSCs-CM induced up-regulation of type I collagen and
MMP-1, promoted the migration of skin fibroblasts, and
thereby promoted the wound healing process in vivo. Our data
indicate that TGF-ß1-treated ADSCs-CM will be a component
for a wound healing accelerating agent.
Keimyung Author(s)(Kor)
조재위
이규석
Publisher
School of Medicine
Citation
JAE-WE CHO et al. (2010). TGF-ß1-treated ADSCs-CM promotes expression of
type Ⅰ collagen and MMP-1, migration of human skin
fibroblasts, and wound healing in vitro and in vivo. International Journal of Molecular Medicine, 26(6), 901–906. doi: 10.3892/ijmm_00000540
Type
Article
ISSN
1107-3756
Source
https://www.spandidos-publications.com/ijmm/26/6/901
DOI
10.3892/ijmm_00000540
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35963
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Dermatology (피부과학)
3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
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