Muscular cell proliferative and protective effects of N-acetylcysteine by modulating activity of extracellular signal-regulated protein kinase

Authors
Geun-Ho KimDae-Kyu SongChi-Heum ChoSun Kyun YooDae-Kwang KimGi-Young ParkSeong-il SuhByeong-Churl JangJeong-Geun Lim
Department
Dept. of Neurology (신경과학); Dept. of Obstetrics & Gynecology (산부인과학); Dept. of Medical Genetics (의학유전학); Dept. of Rehabilitation Medicine (재활의학); Dept. of Microbiology (미생물학); Dept. of Physiology (생리학); Dept. of Molecular Medicine (분자의학); Institute for Medical Science (의과학연구소)
Issue Date
2006
Citation
Life Science, Vol.79(7) : 622-628, 2006
ISSN
0024-3205
Abstract
N-acetylcysteine (NAC), an antioxidant and a precursor of glutathione, is currently in clinical use for various pathological conditions. No data is available as to the relationship between NAC and muscular cell proliferation or muscular degenerative disease. In this study, we assessed the effect of NAC on growth of L6 myoblasts, a rat skeletal muscle cell line, under normal or bupivacaine-treated condition. Of interest, under normal growth conditions, NAC treatment concentration-dependently increased viability, cell number, and DNA incorporation of L6 cells. Remarkably, NAC treatment for 12 to 24 h led to increased phosphorylation of ERKs, a family of mitogen-activated protein kinase known to involve in cell proliferation, in L6 cells, and specific inhibition of ERKs by PD98059, a selective inhibitor of ERKs, greatly abolished the ability of NAC to increase the number of L6 cells. More importantly, pretreatment with NAC effectively blocked decrease in the number and ERKs phosphorylation in L6 cells induced by the exposure of bupivacaine, a local anesthetic with myotoxicity. These results collectively suggest that NAC has muscular cell proliferative and protective effects and the effects by NAC appear to be, in part, mediated via increase in ERKs activation.
Keywords
NACL6 myoblastsBupivacaineERKs
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/36300
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Neurology (신경과학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Obstetrics & Gynecology (산부인과학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Medical Genetics (의학유전학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Rehabilitation Medicine (재활의학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
1. Journal Papers (연구논문) > 3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
Keimyung Author(s)
임정근; 조치흠; 김대광; 박기영; 서성일; 송대규; 장병철
Full Text
https://www.sciencedirect.com/science/article/pii/S0024320506001457?via%3Dihub
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