활성 신경교세포의 Nitric Oxide 생성증가에 의한 망간의 세포독성

Abstract

Background : Glial cells are generally known to support normal neuronal functions tightly regulating the extracellular environment and providing energy substrates such as glucose. Therefor, dysfunction or loss of glial cells will lead to neuonal death. Since manganese (Mn2+) is known to be sequestered in glial cells, we investigated whether nitric oxide (NO) production in the activated glial cells is potentiated by Manganese and the relationship between increased NO production and Manganese cytotoxicity of glial cells. Methods : Manganese toxicity was assessed by morphological examination and by measuring the release of lactate dehydrogenase. Result : Neither a LPS nor a MnCl2 altered the viability of glial cells. A 24 hr stimulation both LPS and MnCl2, however, markedly potential the manganese-induced death of glial cells. Conclusion : It is concluded that manganese could induce sustained production of neurotoxic nitric oxide by the activated glial ceels and manganese-induced cytotoxicity is partially mediated by potentiation of LPS- induced nitric oxide in the glial cell culture model.