고지방식이가 장조직의 지질상 및 지질합성에 미치는 영향

Abstract

Effect of high lipid diet and its composition on lipid profile and lipid biosynthesis was investigated in Sprague-Dawley rats weighing 60-65gm. The animals were divided into three groups and fed 3 different fat diets: 12% calorie control diet, 45% calorie corn oil diet, and 45% calorie butter fat diet. The diets were fed to the rats ad libitum for 12 weeks. And lipid profile and incorporation of acetate-??C into iontestinal lipid were investigated. Mean small intestinal lipid levels in mg/100ml for the control group were 15.2±1.5; triacylgcerol, 2.6±0.5; phospholipid, 1.04±0.14; cholesterol, and 20.4±5.9; total lipid. The increase of lipid content due to long term administration of high lipid diet was more prominent in animal fat (45% calorie butter fat) diet group than in plant fat (45% corn oil) diet group. In 45% calorie-butter fat diet feeding, phospholipid was increased 2-fold and triacylglycerol 1.5-fold but total cholesterol did not show significant increase. On the otherhand, incorporation of radioactive acetate into intestinal tissue revealed maximum level at 3 hours afte administration in all group, and maximum incorporation rate of both plant and animal fat diet group did not show significant difference but slightly decreased than control normal diet group (abort 15%). The result indicated that long-term feeding of animal diet composed 45% calorie butter fat, slightly increases small intestinal levels of triacylglycerol and phospholipid but plant lipid diet do not affect the lipid profile and synthesis of lipid in intestinal tissue.