염색체연구에 있어서 Q-band와 G-band의 차이

Abstract

The cause of the appearance of chromosome bands may be many-fold. Four factors have been found to be of particular importance ； The occurrence of repetitive DNA, differences in the base composition of DNA, differences in the protein components and differences in the degree of packing of the DNP-complex. These factors may of course mutually related. Moreover, All these factors may influence the disposition of the DNA to denature and reassociate under certain conditions, which may be an important factor in producing chromosome bands.

The authors attempted to search these mechanism ： a series of thermal’ 2xSSC, trypsin and pH 9 were utilized to investigate for the G-band and quinacrine, for the Q—band.

The Y-chromosome was the most brilliant and was easily distinguished between G-group disorder chromosome, It seems, as for now, that Q-band is more favorable for male-sex chromosomal patients than Giemsa band. For the G-band, authors may propose the treatment 2xSSC plus trypsin in cytogenetic laboratory.