Chlorambucil이 수컷 생쥐의 생식세포에 미치는 영향

Abstract

The present study was performed to investigate the effects of chlorambucil on the reproductive cells of male mice. The animal was treated with daily injection of chlorambucil (16mg/kg) for periods up to 35 days. The morphological changes in reproductive cells of male mice were observed with light microscope after routine preparation. The results are summarized as follows : 1. Testis, epididymis, prostate, and body weight were increased in control groups, however in the experimental groups, these were severly decreased. 2. The diameter of seminiferous tubule and the height of germinal epithelium cell were decreased, and more numbers of spermatogonia A, spermatogonia B, spermatocyte, spermatid, and spermatozoa were severely decreased. After 35 days treated with chlorambucil, almost all spermatozoa were lost in seminiferous tubules. 3. During the process of treatment, the frequency of cell debris, vacuolations, piknotic nuclei, and multinucleated giant cells were markedly appeared in the germinal epithelial cells. 4. The severe hyperplasia of Leydig cell was revealed, however, the Sertoli cell was not affected with treatment. These results indicate that the chlorambucil causes ultimate lesions for the production of reproductive cell in all stage of the spermatogenesis.