간흡충 탈양유충의 대사산물로 감작한 햄스터 복강삼출세포의 면역이입

Abstract

In order to evaluate the role of peritoneal exudate cells (PEC) and serum in the transfer of immunity againnst Clonorchis sinensis, the goldenhamsters were sensitized which the metabolite of excysted metacercaria of C. sinensis. The donor hamsters(DH) were divided into two groups. One group (Group Ⅰ) was sensitized with two injections of the admixture of the metabolic products of the excysted C. sinensis metacercariae and Freund's incomplete adjuvant into footpads at two weeks interval, and the other group (Group Ⅱ) injected intraperitoneally with the admixture. Two weeks after sensitization, the PEC and serum were collected from the DH under deep anesthesia. Recipient hamsters (RH) were divided into 6 groups. The hamsters of Group 1 were injected intraperitoneally (IP) with 5×10?? PEC from Group ⅠDH, those of Group 2 were injected IP with 1.0ml of serum from Group Ⅰ DH, those of Group 3 were injected IP with 5×10?? PEC from Group Ⅱ DH, and those of Group 4 were injected IP with 1.0ml of serum from Group Ⅱ DH. The hamsters of Group 5 were injected IP with 5×10?? PEC and those of Group 6 were administered IP with 1.0ml of serum from non-sensitized controls. Seven days after primary sensitization, RH were challenged orally with 20 metacercarae and Eggs per Gram (EpG) of fecal samples were counted from 13 to 49 days after challenge. The RH were per Gram (EpG) of fecal samples were counted from 13 to 49 days after challenge. The RH were killed 50 days after challenge, and the transfer of immunity to RH was estimated by significant differences in mean worm burdens and plaque forming cells per spleen between sensitized and nonsensitized groups. The eggs of C. sinensis appeared in the 14 days in the 14 days after challenge in Groups 2, 4, 5 and 6, and in the 15 days in Groups 1 and 2 by the formalin-ether sedimentation, and EpG noted in the 15 and 16 days by Stoll's egg counting techniques. No significant difference was observed in EpG between the sensitized groups and the non-sensitized controls. The hamsters given sensitized PRC harbored fewer flukes than the control groups. The differences in mean worm burdens between the groups given PEC and the control groups were significant by the paired t-test. Whereas no significant differences were found in mean worm burdens between the groups given immune serum and the control group. There were more plaque forming cells in the groups given immune serum than in those given sensitized PEC. However, no plaque froming cells wee encountered in the non-sensitized groups.