녹농균의 항균제내성 및 Pyocin형

Abstract

A total of 94 strains of Pseudomonas aeruginosa were isolated from pus(39), urine(33), sputum(11), and other clinical specimens, and tested for antimicrobial susceptibility to 11 drugs and studied the distribution of pyocin types for epidemiological evaluatioin. Cefoperazone(Cz), cefotaxiime(Ct), moxalactam(Mx), norfloxacin(Nf), and ciprofloxacin(Cf) showed higher antimicrobial activities than other 6 drugs that showed the resistance frequency over 50%. Minimal inhibitory concentration needed to inhibit 90% of straiins(MEC90%) was lowest in Cf. MIC90% of NF, rifampin, Cz, Mx, and Ct were 14.4 to 44.㎍/ml, but other drugs were over 128㎍/ml or more. All strains were found to be resistant to more than one drug and 41 strains were multiply resistant to more than 5 drugs. Pyocin typings as one of the methods for epidemiological fingerprinting of P. aeruginosa were performed through the method of Gillies and Govan. The most predominant type was 11A which occupied 24 strains(25.5%) and followed by 34A(20.2%) and 44A(19.1%). Strains classified into 34A showed higher resistance frequency to drugs of gentamiciin, amikacin, tobramyciin and carbenicillin than those of 11A and some of other types. The pyocin types of two strains which were resistant to Cz were 44A and 74A.