Ethanol중독 흰쥐에서 총담관결찰이 간의 Glutathione S-Transferase, Glutathione Peroxidase 및 Glutathione Reductase 활성에 미치는 영향

Abstract

This study was made to see the effect of common bile duct ligation on liver glutathione S-transferase(GST), glutathione peroxidase (GSH-Px) and glutathione reductase (GR) activities in rats suffering from acute and chronic intoxication of ethanol. For chronic intoxication of ethanol, the rats were fed 5% (v/v) ethanol instead of water for 60 days. Common bile duct of the same group of rats were ligated with ethanol constantly being fed. The rats were then killed on the 1st, 2nd, 3rd, 7th and 14th days of the procedure to measure the cytosolic, mitochondrial and microsomal GST, and cytosolic GSH-Px activities of the liver. The liver GR activities were also measured. For acute intoxication of ethanol, 4g of ethanol were administered orally per kg of body weight as a single dose. The rats were killed at the 1.5th and 24th hours of the procedure for study. On the 14th day following common bile duct ligation, the rats were acutely intoxicated with ethanol to be killed at the 1.5th and 24th hours for measuring the activities of the above enzyme. The rats liver cytosolic and microsomal GST activities showed slight increase in chronically ethanol intoxicated group but the mitochondrial GST activities did not increase in this group. The rats liver cytosolic, mitochondrial and microsomal GST activities showed no significant changes in acutely ethanol intoxicated groups. In terms of rats liver GR and liver cytosolic GSH-Px activities, no significant changes were shown in either chronically ethanol intoxicated groups or acutely ethanol intoxicated groups. The groups that received common bile duct (CBD) ligation after being chronically intoxicated with ethanol showed considerable decrease in the liver cytosolic GST activities. However, the activities showed a less degree than groups of CBD ligation. The liver mitochondrial GST activities of the CBD ligation groups showed slight decrease at the 14th day of the ligation after chronic ethanol intoxication showed significant decrease at the 2nd, 3rd, 7th and 14th days following the operation. The liver microsomal GST activities of the CBD ligation groups showed remarkable increase at the 7th and 14th days of the ligation. But the activities showed no significant increase in the groups with the ligation following the chronic ethanol intoxication. The groups that received CBD ligation after being chronically intoxicated with ethanol showed considerable increase at the 2nd, 3rd, 7th and 14th days following the operation in the liver GR activities. On the other hand, the liver cytosolic GSH-Px activities showed significant increase at the 14th days after the ligation. However, the activities showed a far less degree on the same time points than the groups only with the CBD ligation. At the 1.5th and 24th hours following the acute intoxication with ethanol which was done after 14 days of the CBD ligation, the rats showed less remarkably decrease in the liver cytosolic GST and GSH-Px activities than the group only with the 14th day following CBD ligation. The liver microsomal GST and liver GR activities, however, showed considerable increase at the 1.5th and the 24th hours following the acute intoxication with ethanol which was done after 14 days of the CBD ligation. But the activities showed a less degree than group with the 14th day following CBD ligation. At the 1.5th and 24th following the acute intoxication with ethanol which was done after 14 days of the CBD ligation, the liver mitochondrial GST activity decreased significantly, and the same was seen in the group with the 14th day following CBD ligation.