Ethanol중독 흰쥐에서 총담관결찰이 혈청 및 간의 Aspartate Aminotransferase활성에 미치는 영향

Abstract

This study was intended to observe the effect of common bile duct ligation on serum and liver aspartate aminotransferase (AST) activities in rats suffering from acute and chronic intoxication of ethanol. For chronic intoxication of ethanol, the rat were fed 5%(v/v) ethanol instead of waster for 60days. Common bile duct of the same group of rats were ligated with ethanol constantly being fed. The rats were killed on the 1st, 2nd, 3rd, 7th and 14th days of the procedure to measure the cytosolic and mitochondrial AST activities of the liver. The serum AST activities were also measured. For actue intoxication of ethanol, 4g of ethanol were administered orally per kg of body weight as a single dose. The rats were killed at the 1.5th and 24th hours of the procedure for study. On the 14th day following common bile duct ligation, the rats were acutely intoxicated with ethanol to be killed at the 1.5 and 24th hours for measuring the activities of the ;above enzymes. In terms of rats liver cytosolic and mitochondrial AST activities, no significant increase was shown in either chronically intoxicated ethanol group or acutely intoxicated ethanol group. The liver cytosolic AST activity of the group of common bile duct ligation after chronic ethanol intoxication showed slight decrease at the 14th day after the ligation. The liver mitochondrial AST activity of the group of common bile duct ligation after chronic ethanol intoxication showed marked decrease on the 14th day of the ligation. And the groups of acute intoxication with ethanol which was done after 14 days ofthe common bile duct ligation, the rats showed considerable decrease in the activities. The activities, however, both the groups that recieved the common bile duct ligation after being chronically intoxicated with ethanol which was done after 14 days of the common bile duct ligation showed far less decreases on the same time points than the group only with the common bile duct ligation. The serum AST activity showed slight increase in acutely intoxicated ethanol group. On the other hand, both the common bile suct ligation groups and the one that received the same ligation afterbeing chronically intoxicated with ethanol showed remakable increase in the activities. But the activity of the groups that received the common bile duct ligation after chronical ehtanol intoxication showed far more significant increases on the 7th and 14th days than the groups only with the common bile duct ligation, considerable activity increases were also shown when the groups were acutely intoxicated with ethanol after the ligation.