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투과 및 주사전자현미경 영역에서 면역금표지법

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Author(s)
권건영
Keimyung Author(s)
Kwon, Kun Young
Department
Dept. of Pathology (병리학)
Institute for Medical Science (의과학 연구소)
Journal Title
Keimyung Medical Journal
Issued Date
1996
Volume
15
Issue
1
Keyword
Post-embedding methodSilver-enhancingTransmission immunoelectron microscopyScanning immunoelectron microscopy
Abstract
Immunocytochemistry at the electron microscopic level has allowed the distribution of antigens in the cellular system to be examined. In transmission immunoelectron microscopy, two different approaches have been used, i. e., preembedding or
post-embedding methods, depending on whether labeling is carried out before or after the embedding process, respectively. The good preservation and expression of the antigenic molecules in the tissues are most important in the ultrastructural
immunocytochemistry. A low temperature resin, Lowicryl K4M for postembedding method is frequently used in many immunoelectron microscopic laboratories. Periodate-lysine-paraformaldehyde(PLP) fixed tissues are dehydrated and embedded at -20℃ in a
hydrophilic resin, Lowicryl K4M. The resin is easy to section and a good embedding material for preservation of antigenicity in the tissues. Immunogold labelling technique marks localization of antigenic molecules in the subcellular level.
In the scanning immunoelectron microscopy, a silver-enhancing technique can well express the antigenic immunogold particles using smaller gold probes(<15nm) in the standard scanning electron microscopy.
Alternative Title
Immunogold
Labelling in the Transmission and Scanning Electron Microscopy
Keimyung Author(s)(Kor)
권건영
Publisher
Keimyung University School of Medicine
Citation
권건영. (1996). 투과 및 주사전자현미경 영역에서 면역금표지법. Keimyung Medical Journal, 15(1), 96–104.
Type
Article
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/14968
Appears in Collections:
2. Keimyung Medical Journal (계명의대 학술지) > 1996
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
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