급성 주정 중독 흰쥐에서 간의 Aryl Sulfotransferase와 UDP-Glucuronosyltransferase 활성에 미치는 총담관 결찰의 영향

Abstract

The activities of aryl sulfotransferase (AST) isozymes Ⅰ-Ⅱand Ⅲ-Ⅳand UDP-glucuronosyltransferase (UDP-GT) were measured in rats with acute ethanol intoxication after common bile duct(CBD) ligation to investigate the adverse effects of alcohol ingestion to the liver under the hepatobiliary disease. Serum AST Ⅰ-Ⅱ and AST Ⅲ-Ⅳ activities in CBD ligated rats combined with acute ethanol intoxication showed greater increases than that in CBD ligation alone. These results indicate that the leak of hepatic AST isozymes into blood is facilitated in CBD ligated rats combined with acute ethanol intoxication than in CBD ligation alone due to aggravated hepatic membrane damage. Liver mitochondrial AST Ⅰ-Ⅱ and AST Ⅲ-Ⅳ activities in CBD ligated rats combined with acute ethanol intoxication showed less increases than that in CBD ligation alone. However, liver microsomal UDP-GT activity in CBD ligated rats combined with acute ethanol intoxication showed a more decrease than that in CBD ligation alone. On the other hand, when acute ethanol intoxication was combined with CBD ligation, the values of Vmax of the liver mitochondrial AST Ⅰ-Ⅱ, AST Ⅲ-Ⅳ and liver microsomal UDP-GT decreased significantly than that in CBD ligation alone. However, the values of Km of above hepatic enzymes did not change. Viewed from these results, when acute ethanol intoxication was combined with cholestasis, these enzymes in the liver seem to be decreases their biosynthesis than that in cholestasis. According to all of the results, if ethanol intoxication is combined with cholestasis, the hepatic damage is aggravated.