Wizard Plus Maxipreps Kit를 이용한 Plasmid DNA의 분리법의 응용

Abstract

Since the development of molecular biological techniques, the plasmid DNA became indispensable in the research laboratories. Conventionally, the plasmid DNA was purified using CsCl and over 40 hours of high speed centrifugation. To overcome these problems, several manufactures have developed kits for purifying plasmid DNA based on affinity chromatography, however, the yield of single preparation of plasmid DNA was not sufficient for experiments such as gene therapy, gene delivery using liposome, or cDNA vaccination. In this technical report, by precipitating DNA using ethanol and re-using column, we have developed a simple method for preparing several miligrams of palsmid DNA from 300 ml of over night bacterial culture using Wizard Plus Maxipreps Kit.