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Wizard Plus Maxipreps Kit를 이용한 Plasmid DNA의 분리법의 응용

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Author(s)
이영재한인숙
Journal Title
Keimyung Medical Journal
Issued Date
1998
Volume
17
Issue
2
Abstract
Since the development of molecular biological techniques, the plasmid DNA became indispensable in the research laboratories. Conventionally, the plasmid DNA was purified using CsCl and over 40 hours of high speed centrifugation. To overcome these problems, several manufactures have developed kits for purifying plasmid DNA based on affinity chromatography, however, the yield of single preparation of plasmid DNA was not sufficient for experiments such as gene therapy, gene delivery using liposome, or cDNA vaccination. In this technical report, by precipitating DNA using ethanol and re-using column, we have developed a simple method for preparing several miligrams of palsmid DNA from 300 ml of over night bacterial culture using Wizard Plus Maxipreps Kit.
Alternative Title
Superscale Plasmid DNA
Preparation Using Wizard Plus Maxipreps Kit®
Publisher
Keimyung University School of Medicine
Citation
이영재 and 한인숙. (1998). Wizard Plus Maxipreps Kit를 이용한 Plasmid DNA의 분리법의 응용. Keimyung Medical Journal, 17(2), 136–140.
Type
Article
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/15074
Appears in Collections:
2. Keimyung Medical Journal (계명의대 학술지) > 1998
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