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개회충 성충항원을 이용한 실험적 토끼 개회충증 혈청 항체가의 측정

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Author(s)
주종윤김문규정명숙
Keimyung Author(s)
Joo, Chong Yoon
Department
Dept. of Medical Genetics (의학유전학)
Institute for Medical Science (의과학 연구소)
Journal Title
Keimyung Medical Journal
Issued Date
1998
Volume
17
Issue
2
Keyword
ELISAImmunoblotTbxocara canisToxocariasis
Abstract
Toxocariasis is produced by the migration of the Toxocara canis larvae into the extra-intestinal tissues of unnatural hosts or natural hosts under suitable conditions. Generally, serodiagnosis and treatment may be difficult in this situation. The present study was performed to observe the specifically reacting antigenic bands in crude extract of Toxocara canis adult worm and its reaction in the serum IgG antibody obtained from experimentally infected rabbits.
After homogenization, the homogenate was extracted at 4℃ for 2 days and centrifuged at 20,000 x g for 1 hour. The supernatant was used as the antigen. Infected sera were obtained from the rabbits infected with Toxocara canis embryonated eggs. The control sera were obtained from non-infected rabbits.
The ELISA showed that serum levels of IgG antibody against crude antigen began to increase from 10 to 14 days after the infection.
SDS-PAGE profile showed complex protein bands ranging 6.5 to 200 kDa.
Of them 110, 62.5, 48, 17.5, and 13.5 kDa components were prominent bands.
according to the immunoblot data, the serum antibody recognized major protein bands with molecular weight of 110, 101, 72, 62.5, 48, 42, 32.5, and 13.5 kDa respectively. The 110, 72,62.5, and 48 kDa bands reacted even in non-infected control sera. Cross reactions by immunoblot were also observed with the sera from infected and immunized with Trichinella and Anisakis.
The proteins of 110 and 42 kDa showed as common antigens.
In conclusion, Toxocara canis adult worm antigen represents an antigen that can be used in the diagnosis of toxocariasis. And the 101, 32.5, and 13.5 kDa band were specific antigenic proteins.
Alternative Title
Determination of
antibody Level in Experimental Rabbit Toxocariasis Induced by Crude Worm Antigen
Keimyung Author(s)(Kor)
주종윤
Publisher
Keimyung University School of Medicine
Citation
주종윤 et al. (1998). 개회충 성충항원을 이용한 실험적 토끼 개회충증 혈청 항체가의 측정. Keimyung Medical Journal, 17(2), 202–212.
Type
Article
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/15080
Appears in Collections:
2. Keimyung Medical Journal (계명의대 학술지) > 1998
1. School of Medicine (의과대학) > Dept. of Medical Genetics (의학유전학)
3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
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