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재조합 아데노 바이러스 생산을 위한 효과적인 Shuttle 벡터의 개발과 검증

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Affiliated Author(s)
박종구
Alternative Author(s)
Park, Jong Gu
Journal Title
Keimyung Medical Journal
Issued Date
1998
Keyword
FcyreceptorRecombinant adenovirusShuttle vector
Abstract
Recombinant adenovirus is an attractive vehicle to deliver a foreign gene for the virus can infect many cell types including non-dividing cells. Production of a recombinant adenovirus requires co-transfection of a shuttle vector which harbors a gene of interest and a rescue vector. Adenoviral shuttle vectors currently in use contain limited numbers of cloning sites for insertion of a foreign gene. They also lack a promoter and a signaling sequence for the poly(A) tail. Therefore, we set out to construct two adenoviral shuttle vectors, pAd-YC and pAd-YC2, in an effort to facilitate cloning and expression of foreign genes. pAd-YC contains the CMV promoter and multiple cloning sites but without a poly(A) signal sequence. pAd-YC2 contains the CMV promoter, the multiple cloning sites and the poly(A) signal sequence. The functionality of pAd-YC and pAd-YC2 was tested by cloning and expression of a chimeric Fcγreceptor cDNA and the β-galactosidase gene. Fcγreceptor expression was confirmed by binding of opsonized erythrocytes to COS-1 infectants, and β-galactosidase expression was assayed for X-gal staining. The results demonstrate the functional efficacy of the two shuttle vectors. We have since successfully constructed several recombinant adenoviruses by employing the two versatile shuttle vectors.
Alternative Title
Development of
Convenient Shuttle Vectors for Generation of Recombinant Adenoviruses
Department
Dept. of Molecular Medicine (분자의학)
Institute for Medical Science (의과학 연구소)
Publisher
Keimyung University School of Medicine
Citation
박종구. (1998). 재조합 아데노 바이러스 생산을 위한 효과적인 Shuttle 벡터의 개발과 검증. Keimyung Medical Journal, 17(4), 477–486.
Type
Article
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/15108
Appears in Collections:
2. Keimyung Medical Journal (계명의대 학술지) > 1998
1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
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