계명대학교 의학도서관 Repository

담즙산 부하에 의한 흰쥐 간의 Cholinesterase의 합성 억제

Metadata Downloads
Author(s)
박소경곽춘식
Keimyung Author(s)
Kwak, Chun Sik
Department
Dept. of Biochemistry (생화학)
Institute for Medical Science (의과학 연구소)
Journal Title
Keimyung Medical Journal
Issued Date
1999
Volume
18
Issue
2
Keyword
Bile duct obstructionCholinesteraseCholedocho-caval shuntTaurocholic acidTauroursodeoxycholic acid
Abstract
The possible mechanism of decrease of cholinesterase activity in cholestatic liver and serum was studied. Rats were divided into eight groups: Normal, sham operated control, bile duct obstruction (BDO) alone (BDO group), BDO plus taurocholic acid (TCA) injection (BDO plus TCA group), BDO plus tauroursodeoxycholic acid (TUDCA) injection (BDO plus TUDCA group). choledocho-caval shunt (CCS) operation (CCS group), CCS plus TCA injection (CCS plus TCA group), and CCS plus TUDCA injection (CCS plus TUDCA group). Cholinesterase activity was determined in the serum and liver cytosolic, mitochondrial and microsomal preparations isolated from the above experimental rats. The values of Km and Vmax in this hepatic enzyme were measured. The activity of liver microsomal cholinesterase showed a significant decrease in both the CCS and the BDO groups. And the activity of serum cholinesterase showed a marked decrease in both the CCS and the BDO groups. However, the cholinesterase activity in the serum and liver microsomal preparation fell more rapidly in the BDO group than the CCS group. Cholinesterase activities in liver cytosolic and microsomal preparations, and its Vmax values decreased significantly in both the CCS plus TCA group, and the BDO plus TCA group than that of each control group, such as CCS and BDO groups. On the other hand, the values of Km of the liver cytosolic and microsomal cholinesterase did not change in all the experimental groups. Serum cholinesterase activity decreased significantly in both the CCS plus TCA group and the BDO plus TCA group than that of each control group. However, these serum and hepatic enzymes activities did not change in both the CCS plus TUDCA group and the BDO plus TUDCA group. The above results suggest that TCA repress biosynthesis of cholinesterase in the liver. And the lowering of the serum enzyme activity is thought to be caused by a decrease of biosynthesis of this hepatic enzyme, which causes the enzyme to leak into the blood in small quantity.
Alternative Title
Repression of Rat Hepatic Cholinesterase by Bile Acid Load
Keimyung Author(s)(Kor)
곽춘식
Publisher
Keimyung University School of Medicine
Citation
박소경 and 곽춘식. (1999). 담즙산 부하에 의한 흰쥐 간의 Cholinesterase의 합성 억제. Keimyung Medical Journal, 18(2), 204–217.
Type
Article
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/15138
Appears in Collections:
2. Keimyung Medical Journal (계명의대 학술지) > 1999
1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
공개 및 라이선스
  • 공개 구분공개
파일 목록

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.