총담관을 결찰한 흰쥐 혈청 및 간의 Isocitrate Dehydrogenase활성도와 총담즙산의 농도

Abstract

A study was made on the changes of NADP+- and NAD+ dependent isocitrate dehydrogenase (NADP+-ICD, NAD+-ICD) activities in cholestatic rat liver. The cytosolic and mitochondrial NADP+-ICD and mitochondrial NAD+-ICD activities were determined in the cholestatic rat liver induced by common bile duct ligation over a period of forty two days. And the activities of NADP+- and NAD+-ICD in the serum were measured. The concentrations of total bile acids in their serum and liver cytosolic fraction were also measured. The cytosolic and mitochondrial NADP+-ICD activities of the cholestatic rat liver each showed a marked decrease between the 1st and the 42nd day and the 12th hour and the 42nd day respectively after the ligation. However, the mitochondrial NAD+-ICD activity in the cholestatic liver markedly increased between the 2nd and the 42nd day after the ligation. On the other hand, the NADP+-ICD activity of the serum markedly increased between the 12th hour and 3rd day after the ligation. That of the serum NAD+-ICD also showed a marked increase between the 1st and the 7th day after the ligation. The concentrations of total bile acids in the cholestatic liver cytosolic fraction showed a marked increase between the 12th hour and the 7th day after the ligation. That of total bile acids in the serum showed a marked increase throughout the experiments. Viewed from above results, the NADP+-ICD in the liver with cholestasis seems to be the decreasing enzyme. But the NAD+-ICD in the cholestatic liver thought to be the increasing enzyme in its activity. Especially, when the cholestasis occurred, the activities of these enzymes of serum are higher than those of the controls because of increased permeability secondary to liver cell necrosis, which causes the enzymes to leak into the blood in great quantity.