냉각식염수로 분리뇌관류가 토끼 해면구 세포 외액의 Glutamate; Glycine 농도에 미치는 영향

Abstract

Background: When ischemia reduces blood supply, hypothermia remains the sine qua non for reducing demand. An alternative to whole body deep hypothermia is an isolated cerebral hypothermia via perfusion of cooled blood through one internal carotid artery. The goal of this study was to evaluate the effect of isolated cold hemisphere perfusion during the cerebral ischemia on periischemic glutamate and glycine concentrations. Methods: Experiments were carried out with animal(New Zealand White rabbit). Rabbit was to perfuse a saline solution into carotid artery with a cold saline(20℃). Animals were devided into two groups. In experimental group, the cerebral ischemia was produced by a combination of carotid artery saline perfusion and systemic hypotension to a mean arterial blood pressure of 40 mmHg for 10 minutes and control group received no perfusion during the ischemia. Results: Glutamate and glycine concentrartions during ischemia in the experimental group showed increased significantly compared to control group(p<0.05). Conclusions: Cerebral cold saline perfusion during the ischemia did not prevent increase of glutamate and glycine during ischemia. These results showed hypothemia induced by selective brain perfusion may not be one of the most effective ways to protect brain from the ischemia.