만성 주정 중독 쥐에서 총담관 결찰이 간의 Aryl Sulfotransferase I,II Isozyme의 활성에 미치는 영향

Abstract

The drinking hazard in hepatobiliary disease was studied by the assays of liver cytosolic, mitochondrial, and microsomal aryl sulfotransferase Ⅰ, Ⅱ isozyme, in cholestasis induced by common bile duct (CBD) ligation after chronic ethanol intoxication with rats. The activity of serum aryl sulfotrans-ferase Ⅰ, Ⅱ isozyme was also measured. Liver mitochondrial aryl sulfotransferase Ⅰ, Ⅱ isozyme activity in group of CBD ligation after chronic ethanol intoxication showed a greater decrease between the 3rd and the 14th day after CBD ligation than that in group of CBD ligation alone. However, the cytosolic Ⅰ, Ⅱ isozyme activity did not change. The microsomal aryl sulfotransferase Ⅰ, Ⅱ isozyme activity in the CBD ligation after chronic ethanol intoxication showed a greater decrease between the 7th and the 14th day after CBD ligation than that in the CBD ligation alone. The serum aryl sulfotransferase Ⅰ, Ⅱ isozyme activity in the CBD ligation after chronic ethanol intoxication showed a greater increase between the 2nd and the 14th day after CBD ligation than that in the CBD ligation alone. The Vmax values of the liver mitochondrial and microsomal aryl sulfotransferase Ⅰ, Ⅱ isozymes in the CBD ligation after chronic ethanol intoxication showed a greater decrease at the 14th day after CBD ligation than that in the CBD ligation alone. However, the Km values of the above hepatic enzymes did not change. The results indicate that the biosynthesis of the hepatic aryl sulfotransferase Ⅰ, Ⅱ isozyme is decreasing in chronic ethanol intoxication with cholestasis than in cholestasis alone. The increased activity of the serum aryl sulfotransferase Ⅰ, Ⅱ isozyme in chronic ethanol intoxication with cholestasis than in cholestasis alone reflect that icreased hepatic damages which cause this isozyme to leak into the blood in great quantity.