쥐 재생간에서의 Arylamine N-Methyltransferase 활성도

Abstract

Changes of arylamine N-methyltransferase (AMT) activity during regeneration of rat liver was studied. Cytosolic, mitochondrial and microsomal AMT activities were determined in regenerating rat livers over a period of ten days after 70％ (median and left lateral lobes) partial hepatectomy. The enzyme activity in serum, and Km and Vmax values of the hepatic enzyme were also measured. The activities of cytosolic and mitochondrial AMT were found to be significantly increased between the first and the second day of regenerating rat liver, whereas microsomal AMT increased between the first and the third day after partial hepatectpmy. On the other hand, the serum AMT activity did not change. The Vmax values of the cytosolic, mitochondrial and microsomal AMT in the regenerating rat liver were significantly increased at the second day after partial hepatectomy, however, the Km values of the above hepatic enzymes did not vary in all experimental groups. Therefore, the above results indicate that the biosynthesis of AMT was increased during the regeneration of rat liver.