담즙울체 쥐에서 간의 Glyoxalase Ⅱ 활성도에 미치는 Taurocholate의 정맥 내 투여의 효과

Abstract

The possible mechanisms of decreased glyoxalase Ⅱ (GLO-Ⅱ) activity in cholestatic rat liver was studied. Hepatic GLO-Ⅱ activity was determined from the experimental rats with choledocho-caval shunt (CCS) or bile duct obstruction (BDO). The Michaelis-Menten constant in this hepatic enzyme was also measured. The activity of hepatic GLO-Ⅱ as well as the Vmax value was found to be decreased significantly in both CCS plus taurocholic acid (TCA) injection group, and BDO plus TCA injection group than in each control group such as CCS alone and BDO alone. However, the GLO-Ⅱ activity did not change in both the CCS plus tauroursodeoxycholic acid injection group and the BDO plus tauroursodeoxycholic acid injection group. On the other hand, the Km value of the GLO-Ⅱ did not vary in any experimental groups. Above results suggest that TCA repress the biosynthesis of GLO-II in the liver.