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실험견에서 E2F Decoy 전이가 자가정핵 이식견의 내막과형성에 미치는 영향

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Author(s)
이성억조원현
Keimyung Author(s)
Cho, Won Hyun
Department
Dept. of Surgery (외과학)
Journal Title
Keimyung Medical Journal
Issued Date
2003
Volume
22
Issue
2
Keyword
E2F decoyIntimal hyperplasia
Abstract
One of the leading causes of
coronary and peripheral vascular graft failure is intimal hyperplasia at the vascular anastomotic site. Double-stranded DNA with high affinity for E2F as decoy cis elements may block the activation of genes mediating the cell cycle, suggesting that it could possibly be an effective therapeutic agent for treating intimal hyperplasia. In order to evaluate the effect of E2F decoy in a large animal model, jugular vein to femoral artery interposition grafts in experimental dogs with 15-20 kg body weight were treated, with either heparin, E2F decoy oligodeoxynucleotides (ODN) or mismatched ODN by hemagglutinating virus of Japan (HVJ) -liposome method using pressure-mediated delivery. The animals were divided into four groups, depending on the method of treatment and duration of observation. Group 1 (n= 3): heparin treated, four months after bypass, Group 2 (n= 3): E2F treated, four weeks after bypass, Group 3 (n= 4): E2F treated, and Group 4 (n= 4): mismatched ODN treated, four months after bypass. Computerized planimetry was used to measure intimal and medial cross-sectional surface areas of each vessel. The intima/media area ratios were compared between the groups after bypass surgery. Treatment of grafts with E2F decoy resulted in a significant reduction of intimal thickness after four months (intima/media area ratio= 0.26 ± 0.11) compared with mismatched ODN-treated group (intima/media area ratio= 1.61 ± 0.28) or heparin alone group (intima/media area ratio=1.49 ± 0.29) (p= 0.000). There was no difference between group 2 (0.37 ± 0.32) and 3 (0.26 ± 0.11) and between proximal and distal anastomotic sites. The above results suggest that gene therapy of E2F decoy in vein graft could be a new therapeutic strategy to prolong the graft patency by suppressing the intimal hyperplasia.
Alternative Title
Effect of E2F Decoy
Oligodeoxynucleotides Gene Therapy on
Intimal Hyperplasia in Autogenous Vein Graft of Dogs
Keimyung Author(s)(Kor)
조원현
Publisher
Keimyung University School of Medicine
Citation
이성억 and 조원현. (2003). 실험견에서 E2F Decoy 전이가 자가정핵 이식견의 내막과형성에 미치는 영향. Keimyung Medical Journal, 22(2), 149–160.
Type
Article
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/15276
Appears in Collections:
2. Keimyung Medical Journal (계명의대 학술지) > 2003
1. School of Medicine (의과대학) > Dept. of Surgery (외과학)
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