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장기간 복막투석시 발생하는 섬유화에 대한 TGF-β1 Antisense의 억제 효과

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Affiliated Author(s)
박성배한승엽황은아김현철박관규
Alternative Author(s)
Park, Sung BaeHan, Seung YeupHwang, Eun AhKim, Hyun ChulPark, Kwan Kyu
Journal Title
Keimyung Medical Journal
Issued Date
2004
Keyword
Transforming growth factor-β1
Abstract
Ultrafiltration failure is a major
cause of dropout of long-term peritoneal dialysis (PD), and often related to peritoneal fibrosis. High glucose solution in dialysate has been implicated as a culprit of peritoneal fibrosis in long-term PD patients. We examined the protective effect of TGF-β1 antisense on peritoneal fibrosis. Human peritoneal mesothelial cells (HPMCs) were isolated from omentum and divided into three groups as control, incubated with high glucose, and incubated with high glucose and transfected TGF-β1 antisense groups. In animal experiment, Spraue-Dawley rats were divided into two groups as control PD group and TGF-β1 antisense treated PD groups. The degree of fibrosis were assayed by RT-PCR, H&E stain, immunohistochemistry stain and Masson’s Trichrome stain. The TGF-β1 expression in vitro increased 1.6 times in the group treated with high glucose solution, and was normalized by the addition of TGF-β1 antisense. In the animal model of PD, submesothelial thickening and depositions of collagen were seen under light microscopy. The degree of peritoneal fibrosis increased with the time on PD treatment. The addition of TGF-β1 antisense into the dialysate reduced the deposition of collagen, however, it failed to show the protective effect against peritoneal thickening. In conclusion, the present study demonstrates that high glucose up-regulates TGF-β1 expression in the cultured HPMCs, which is normalized by addition of TGF-β1 antisense. The protective effect of TGF-β1 antisense on peritoneal fibrosis could not be demonstrated in the animal model of PD.
Alternative Title
Effect of TGF- 1
Antisense Oligodeoxynucleotides on Fibrosis of Peritoneum in Peritoneal Dialysis Animal Model and Mesothelial Cells Cultured in High Glucose Media
Department
Dept. of Internal Medicine (내과학)
Dept. of Pathology (병리학)
Kidney Institute (신장연구소)
Publisher
Keimyung University School of Medicine
Citation
이진구 et al. (2004). 장기간 복막투석시 발생하는 섬유화에 대한 TGF-β1 Antisense의 억제 효과. Keimyung Medical Journal, 23(2), 65–76.
Type
Article
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/15322
Appears in Collections:
2. Keimyung Medical Journal (계명의대 학술지) > 2004
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
3. Research Institutues (연구소) > Kidney Institute (신장연구소)
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