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Detection of cyanate, nitrate and carbamylated proteins in the peritoneal dialysate

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Author(s)
Hyun Chul KimSung Bae ParkSung Yeup HanEun A HwangHye Jung ChoiSon Jung NamEunyoung HaYou Hee KimKyo Cheol Mun
Keimyung Author(s)
Kim, Hyun ChulPark, Sung BaeHan, Seung YeupHwang, Eun AhHa, Eun YoungKim, You HeeMun, Kyo Cheol
Department
Dept. of Internal Medicine (내과학)
Dept. of Biochemistry (생화학)
Kidney Institute (신장연구소)
Journal Title
Keimyung Medical Journal
Issued Date
2009
Volume
28
Issue
1
Keyword
CarbamylationCyanateNitratePeritoneal dialysis
Abstract
Cyanate is known to contribute to peritoneal injury with morphological changes in the peritoneum. Against carbamylation by cyanate, some proteins including albumin are known to play a protective role by self-carbamylation. Although many studies about cyanate and carbamylated proteins, there are no reports about the detection of the cyanate and carbamylated protein in peritoneal dilaysate. In this study, the cyanate, nitrate, and the carbamylated protein levels in peritoneal dialysate were measure to determine the existence of the uremic toxins in peritoneal dialysate. Cyanate was determined using by the isocratic size exclusion
chromatography. Proteins such as albumin, globulin, and hemoglobin were carbamylated with sodium cynate. For the determination of the carbamylated protein in peritoneal dialysate, we performed HPLC system. Nitrite concentration was also assessed in the peritoneal dialysate. In this study, cyanate and nitrate were detected in the peritoneal dialysate. However, carbamylated proteins are not detected in the peritoneal dialysate. According to this study, peritoneal damage during PD may caused by continuous contact with uremic toxins including cyanate and nitrate without proper protection such as proteins.
Keimyung Author(s)(Kor)
김현철
박성배
한승엽
황은아
하은영
김여희
문교철
Publisher
Keimyung University School of Medicine
Citation
Hyun Chul Kim et al. (2009). Detection of cyanate, nitrate and carbamylated proteins in the peritoneal dialysate. Keimyung Medical Journal, 28(1), 1–7.
Type
Article
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/15421
Appears in Collections:
2. Keimyung Medical Journal (계명의대 학술지) > 2009
1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
3. Research Institutues (연구소) > Kidney Institute (신장연구소)
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