Cisplatin에 의해 유도된 신장 손상시의 유전자 발현

Abstract

Cisplatin, an antitumor agent widely used in the treatment of a variety of human solid tumors, has nephrotoxicity. To understand the mechanism for the nephrotoxicity, the gene expression patterns of renal tissues were measured. Sprague-Dawley rats received a single intraperitoneal injection of 8 mg/kg of cisplatin and sacrificed 6 days after cisplatin injection. The genes showed significant changes in expression are 111 genes, are associated with a broad range of functional activities, including apoptosis, cell cycle, cell growth/maintenance, immunoresponse, response to stress, signal transduction, transcription, and metabolism. The expression of the fifty two genes was increased, and that of fifty nine genes was decreased. Especially, the expression of the genes associated with apoptosis such as Spp1, Tubb5, Hmox1 and Clu was increased. And the expression of the genes associated with cell cycle such as Pttg1, Ccng1 and Anxa1 was also increasd. These results suggest that cisplatin induce nephrotoxicity by the changes of gene expression involved in apoptosis and cell cycle.