신장에서 cisplatin이 활성산소 대사에 미치는 영향

Abstract

Cisplatin, an antitumor agent widely used in the treatment of a variety of human solid tumors, has nephrotoxicity. To understand the mechanism for the nephrotoxicity, oxidative stress markers levels and activities of antioxidative enzymes were measured. Sprague-Dawley rats received a single intraperitoneal injection of 8 mg/kg of cisplatin and sacrificed 6 days after cisplatin injection. When mitochondria was exposed to cisplatin, the oxygen free radical production was increased depending on the exposure time. Cisplatin treated rats showed the increase of hydrogen peroxide levels and xanthine oxidase activity in serum. However, catalase activity was decreased. The mRNA expression for renal superoxide dismutase was decreased in the cisplatin treated rats. These results suggest that cisplatin induces nephrotoxicity is associated with the production of reactive oxygen species via the changes of the antioxidant enzymes.