역동적 조영증강 자기공명 영상기법을 이용한 동물모델에서의 종양 혈관 투과율에 관한 연구

Abstract

요 약 본 연구에서는 조영제의 약동학적 모델을 이용하여 역동적 조영증강 자기공명영상 데이터를 분석 하는 경우 기존의 조영증강 패턴 분석으로는 알 수 없었던 종양 혈관의 미세구조를 반영하는 혈관투과 율에 대한 정량적 정보를 제공함으로서 향후 종양 의 특성을 파악하는데 매우 유용하게 이용될 수 있 을 것으로 기대된다.

The purpose of this study was to evaluate the feasibility of pharmacokinetic analysis of MR contrast agent in dynamic MR for providing quantitative vascular permeability, which reflects the angiogenesis. The dynamic MR was performed at 10 and 17 days after VX2 tumor implantation on thigh of adult rabbit. The gadopentetate dimeglumine (0.2 mmol/kg) was injected through ear vein. All MR experiments were carried out in 1.5T MR scanner with human extremity coil. Dynamic images were acquired every 17 sec for the first 3 minutes and then every 2 or 3 minutes up to 45 minutes using 2D SPGR sequence. By locating ROI on tumor, the signal intensity was measured for each image and the corresponding time-intensity curve was obtained. The time-intensity curve was fitted with pharmacokinetic model of MR contrast agent using symbolic calculation computer software to yield the fitting parameters such as vascular permeability and EES volume. Ten days after tumor implantation, the tumor size was 2 cm and increased to 5 cm at 17 days. The calculated vascular permeability (Kp↔t/VT) was 0.2584 ± 0.0275 min-1 and EES volume (Ve) was 0.0870 ± 0.0021 at 10 days after tumor implantation.