조골세포에서 시안산에 의한 유전자 발현

Abstract

Renal osteodystrophy(ROD) contributes as a major long-term complication which is associated with high rate of morbidity. The factors contributing to ROD are still incompletely characterized. Cyanate, known as one of the uremic toxins, is derived spontaneously from urea. According to previous studies, cyanate appears to play a role as a trigger for apoptosis by activating proapoptotic signals in osteoblasts. The purpose of this study is to detect the gene expression by cyanate in osteoblasts. Microarray for 4,800 genes to detect gene expression was performed using ROS 17/2.8 cells incubated with 20 mmol/L cyanate. About twenty percent of the genes among 4,800 genes were expressed by cyanate, and most of the expressed genes except the genes which are related stress were inhibited by cyanate. According to these results, cyanate may play a role in the pathogenesis of ROD due to plays as one of the stress factors and the inhibitor for gene expression in osteoblasts.