Glucosamine Inhibits Lipopolysaccharide-Induced Microglial Activation via a Down-Regulation of iNOS Gene
- Author(s)
- Jae Hoon Bae; Hyon Ah Yi
- Keimyung Author(s)
- Bae, Jae Hoon; Yi, Hyon Ah
- Department
- Dept. of Physiology (생리학)
Dept. of Neurology (신경과학)
- Journal Title
- Keimyung Medical Journal
- Issued Date
- 2011
- Volume
- 30
- Issue
- 1
- Keyword
- Glucosamine; Inducible nitric oxide synthase; Lipopolysaccharide; Microglia
- Abstract
- This study investigated the effects of glucosamine on lipopolysaccharide (LPS)-induced cellular
activation, inducible nitric oxide synthase (iNOS) expression in microglia, and the molecular and
signaling mechanisms involved. LPS with 100 ng/mL was used for activation of BV2 microglial cells. LPS-induced mRNA and protein expressions for iNOS and other signaling factors were analyzed by reverse-transcription polymerase chain reaction and Western blot analysis, respectively. Glucosamine attenuated LPS-induced iNOS protein and mRNA expression in doseand time-dependent manners, but did not affect iNOS protein stability. However, the inhibitory
actions of glucosamine on LPS-induced iNOS expressions were independent of nuclear factor-κB
signals and mitogen-activated protein kinases activations. Glucosamine inhibited LPS-induced
phosphorylation of ribosomal p85 S6, translational regulatory protein, which may down-regulate
translation in iNOS mRNA. These results suggest that inhibitory action mechanisms of glucosamine on LPS-induced microglial activation include down-regulation of microglial activator gene iNOS herein.
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