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Glucosamine Inhibits Lipopolysaccharide-Induced Microglial Activation via a Down-Regulation of iNOS Gene

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Author(s)
Jae Hoon BaeHyon Ah Yi
Keimyung Author(s)
Bae, Jae HoonYi, Hyon Ah
Department
Dept. of Physiology (생리학)
Dept. of Neurology (신경과학)
Journal Title
Keimyung Medical Journal
Issued Date
2011
Volume
30
Issue
1
Keyword
GlucosamineInducible nitric oxide synthaseLipopolysaccharideMicroglia
Abstract
This study investigated the effects of glucosamine on lipopolysaccharide (LPS)-induced cellular
activation, inducible nitric oxide synthase (iNOS) expression in microglia, and the molecular and
signaling mechanisms involved. LPS with 100 ng/mL was used for activation of BV2 microglial cells. LPS-induced mRNA and protein expressions for iNOS and other signaling factors were analyzed by reverse-transcription polymerase chain reaction and Western blot analysis, respectively. Glucosamine attenuated LPS-induced iNOS protein and mRNA expression in doseand time-dependent manners, but did not affect iNOS protein stability. However, the inhibitory
actions of glucosamine on LPS-induced iNOS expressions were independent of nuclear factor-κB
signals and mitogen-activated protein kinases activations. Glucosamine inhibited LPS-induced
phosphorylation of ribosomal p85 S6, translational regulatory protein, which may down-regulate
translation in iNOS mRNA. These results suggest that inhibitory action mechanisms of glucosamine on LPS-induced microglial activation include down-regulation of microglial activator gene iNOS herein.
Keimyung Author(s)(Kor)
배재훈
이현아
Publisher
Keimyung University School of Medicine
Citation
Jae Hoon Bae and Hyon Ah Yi. (2011). Glucosamine Inhibits Lipopolysaccharide-Induced Microglial Activation via a Down-Regulation of iNOS Gene. Keimyung Medical Journal, 30(1), 7–16.
Type
Article
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/15514
Appears in Collections:
2. Keimyung Medical Journal (계명의대 학술지) > 2011
1. School of Medicine (의과대학) > Dept. of Neurology (신경과학)
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
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