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Effect of Immunosuppressive Drugs on the Metalloproteinase in the Glioma Cells and Osteoblasts

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Author(s)
Kyo Cheol Mun
Keimyung Author(s)
Mun, Kyo Cheol
Department
Dept. of Biochemistry (생화학)
Journal Title
Keimyung Medical Journal
Issued Date
2014
Volume
33
Issue
1
Keyword
CyclosporineMMP-2MMP-9Tacrolimus
Abstract
The matrix metalloproteinases (MMPs) play a key role in the normal physiology of connective
tissue during development, morphogenesis, and wound healing. Dysregulation of their activity has
been implicated in numerous diseases including encephalopathy and the process of bone loss.
Thus, MMPs may play a role in the encephalopathy and post-transplantation bone disease by
immunosuppressive drugs such as cyclosporine (CsA) and tacrolimus. Gelatin zymography of
MMP-9 and MMP-2 was performed in the glioma cells and osteoblast after CsA or tacrolimus
treatment. Glioma cells or rat osteoblast ROS17/2.8 cells were treated with CsA or tacrolimus to
make final concentration from 2 to 250 μM. After incubation, gelatin zymography of MMP-9 and
MMP-2 was performed. And the density for the MMP bands were measured using luminescent
image analyzer system. Both MMP-9 and MMP-2 activities in the osteoblast cells were
decreased depending on the concentration of CsA or tacrolimus. MMP-2 activity was increased
after CsA or tacrolimus treatment in the glioma cells. However, MMP-9 activities were
decreased after CsA or tacrolimus treatment in the glioma cells. These results indicate that
dysregulation of MMPs in the osteoblast and in the glioma cells by immunosuppressive drugs may
one of the contributing factors in post-transplantation bone disease and in the encephalopathy
by tacrolimus or cyclosporine.
Keimyung Author(s)(Kor)
문교철
Publisher
Keimyung University School of Medicine
Citation
Kyo Cheol Mun. (2014). Effect of Immunosuppressive Drugs on the Metalloproteinase in the Glioma Cells and Osteoblasts. Keimyung Medical Journal, 33(1), 23–27.
Type
Article
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/15625
Appears in Collections:
2. Keimyung Medical Journal (계명의대 학술지) > 2014
1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
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