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도파민성 신경독성에서 산화스트레스의 역할

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Author(s)
강병준김준철여주천정종희박원균
Keimyung Author(s)
Park, Won Kyun
Department
Dept. of Medical Education (의학교육학)
Brain Research Institute (뇌연구소)
Journal Title
Keimyung Medical Journal
Issued Date
2005
Volume
24
Issue
2
Keyword
AntioxidantsDopaminergic neurotoxicityMPP+Oxidative stress
Abstract
MPTP (1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine) and its metabolite MPP+ (1-methyl-4-phenyl pyridium) are specific dopaminergic neurotoxins and induce parkisonism-like symptoms and signs in animal models. This study was designed to evaluate the effect of oxidative stress on MPP+ -induced dopaminergic toxicity and the protective effects of antioxidants. PC12 cells were exposed to the dopaminergic neurotoxicity by adding 0.05-10 mM MPP+ for 1-24 hrs. In the cells treated with 2 mM MPP+, cell number decreased and the neurites were markedly withdrawal and the cell viability decreased almost 50%. Nerve growth factor (NGF)-differentiated PC12 cells showed higher viabilities than untreated PC12 cells in 0.1-10 mM MPP+. N-acetyl cysteine (NAC, 0.1-10 mM) showed significant protective effects against the dopaminergic cytotoxicity with 2 mM MPP+, and led to almost complete recovery to normal cells over 3 mM NAC. L-2-oxothiazoline-4-carboxylate (OTC, 0.1-2 mM) showed slightly increased the viability of PC12 cells against the dopaminergic toxicity with 2 mM MPP+. however there was not a significant recovery comparing with normal cells. L-lipoic acid (0.1-2 mM) showed some protective effects on PC12 cells against thd dopaminergic cytotoxicity with 2 mM MPP+, however they did not show a significant recovery comparing with normal PC12 eclls. In conclusion, it seems that oxidative stress participates as an important mechanism in MPP+-include dopaminergic neurotoxicity nad NAC may be a good neuroprotective agent against MPP+ -induced dopaminergic neurotoxicity.
Alternative Title
Effects of Oxidative Stress on Dopaminergic Neurotoxicity
Keimyung Author(s)(Kor)
박원균
Publisher
Keimyung University School of Medicine
Citation
강병준 et al. (2005). 도파민성 신경독성에서 산화스트레스의 역할. Keimyung Medical Journal, 24(2), 146–155.
Type
Article
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/15969
Appears in Collections:
2. Keimyung Medical Journal (계명의대 학술지) > 2005
3. Research Institutues (연구소) > Brain Research Institute (뇌연구소)
1. School of Medicine (의과대학) > Dept. of Medical Education (의학교육학)
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